Inhibitory Effects of the Natural Product Esculetin on Phytophthora capsici and Its Possible Mechanism
Inhibitory Effects of the Natural Product Esculetin on Phytophthora capsici and Its Possible Mechanism
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天然产物七叶素对辣椒疫霉的抑制作用及其可能机制（在线发表）
DOI：
10.1094/pdis-09-20-2054-re
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发表时间：
2021-06-01
期刊：
影响因子：
4.5
通讯作者：
Chen, Yu
中科院分区：
文献类型：
--
作者：
Wang, Bi;Li, Pirui;Chen, Yu
七叶苷是一种重要的植物源天然产物，具有多种生物活性和用途。辣椒疫霉是一种臭名昭著的植物病原体，能够感染广泛的宿主。本研究评价了七叶壳对辣椒疫霉菌的抑菌活性。利用从中国江苏和山东省不同地理区域采集的108个辣椒疫霉菌株，建立了辣椒疫霉菌对七叶壳的敏感性基线。escuoke的中位有效浓度（EC 50）值范围为2.08至16.46 μ g/ml（平均值为6.87 +/- 2.70 μ g/ml），呈正态分布。此外，七叶枯克还强烈抑制游动孢子的产生和萌发。更重要的是，escuaoke对番茄上的辣椒疫霉具有保护和治疗活性，并且能够限制辣椒疫霉对本氏烟草的早期感染。通过形态学观察、相对电导率和丙二醛（MDA）含量测定，发现七叶克处理导致辣椒疫霉细胞膜损伤。结果还表明，七叶壳可能通过抑制辣椒疫霉DNA和蛋白质的合成而对辣椒疫霉产生不利影响。这些发现将有助于更广泛地评估使用escuaoke控制由P. capsici引起的疾病，并更好地了解其作为潜在杀菌剂的作用方式。
Esculetin is an important plant-derived natural product that has multiple bioactivities and applications. Phytophthora capsici is a notorious plant pathogen capable of infecting a broad range of hosts. In this study, we evaluated the antifungal activity of esculetin against P. capsici. The baseline sensitivity of P. capsici to esculetin was established using 108 isolates collected from various geographical regions in the Jiangsu and Shandong Provinces of China. The median effective concentration (EC50) values for esculetin ranged from 2.08 to 16.46 mu g/ml (mean, 6.87 +/- 2.70 mu g/ml) and were normally distributed. Furthermore, both zoospore production and germination were strongly inhibited by esculetin. Importantly, esculetin exhibited protective as well as curative activities against P. capsici on tomato and was capable of restricting the early infection of P. capsici on Nicotiana benthamiana. We found that the esculetin treatment led to cell membrane damage of P. capsici, as revealed by morphological observations and measurements of relative conductivity and malondialdehyde (MDA). Finally, our results also suggested that esculetin may adversely affect P. capsici by inhibiting its DNA and protein synthesis. These findings will contribute to the broader evaluation of the use of esculetin to control diseases caused by P. capsici and toward a better understanding of its mode of action as a potential fungicide.