Mesityl phenanthroline-modified 2'-deoxyuridine for heteroleptic complexes in metal ion-mediated assembly of DNA.
Mesityl phenanthroline-modified 2'-deoxyuridine for heteroleptic complexes in metal ion-mediated assembly of DNA.
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DOI：
10.1039/c5dt00100e
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发表时间：
2015-04
影响因子：
4
通讯作者：
P. Ensslen;H. Wagenknecht
中科院分区：
文献类型：
--
作者：
P. Ensslen;H. Wagenknecht
合成了一种新的DNA构建单元，该单元以金属离子配体2,9-二-甲基亚基-3-乙基-菲罗啉连接在2'-脱氧尿嘧啶的5位上。在Zn（2+）、Cu（2+）、Fe（2+）和Ni（2+）存在下，用光谱学研究了相应修饰的DNA双链与含有2,2':6',2 " -三吡啶配体的第二DNA双链的配合物形成。变性聚丙烯酰胺凝胶电泳证实了两个不同DNA片段之间的异电性组装的选择性形成。
The synthesis of a new DNA building block that bears the metal ion ligand 2,9-bis-mesityl-3-ethynyl-phenanthroline attached to the 5-position of 2'-deoxyuridine is presented. In the presence of Zn(2+), Cu(2+), Fe(2+) and Ni(2+) the complex formation of an accordingly modified DNA double strand with a second DNA duplex bearing the 2,2':6',2''-terpyridine ligand was studied by optical spectroscopy. The selective formation of heteroleptic assemblies between the two different DNA pieces was evidenced by denaturing polyacrylamide gel electrophoresis.