Membrane interaction between Span 80 vesicle and phospholipid vesicle (liposome): Span 80 vesicle can perturb and hemifuse with liposomal membrane
Membrane interaction between Span 80 vesicle and phospholipid vesicle (liposome): Span 80 vesicle can perturb and hemifuse with liposomal membrane
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DOI：
10.1016/j.colsurfb.2012.12.022
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发表时间：
2013-06-01
影响因子：
5.8
通讯作者：
Umakoshi, Hiroshi
中科院分区：
文献类型：
--
作者：
Hayashi, Keita;Tatsui, Tsuyoshi;Umakoshi, Hiroshi
我们重点研究了Span 80囊泡与磷脂囊泡（脂质体）之间的相互作用。将Span 80囊泡与磷脂囊泡（脂质体）混合，作为质膜的简化模型。钙黄蛋白渗漏实验表明，Span 80囊泡与脂质体的相互作用会扰乱脂质体膜。在基于荧光共振能量转移的实验中，观察了Span 80囊泡与脂质体之间的脂质混合。上述现象也得到了laurdan荧光光谱分析的支持，导致脂质体与Span 80囊泡混合后膜性质发生变化。这些结果表明，Span 80囊泡可以很容易地与脂质体膜相互作用和半融合，这取决于Span 80囊泡的膜性质、组分的“柔性”和“分子结构”。(C) 2012 Elsevier B.V.版权所有
We have focused on the interaction between the Span 80 vesicle and phospholipid vesicle (liposome). The Span 80 vesicle was mixed with a phospholipid vesicle (liposome), used as a simplified model of plasma membrane. From calcein leakage experiments, it was revealed that the interaction between the Span 80 vesicles and the liposomes could perturb the liposome membrane. In the experiments based on fluorescence resonance energy transfer, the lipid mixing between Span 80 vesicle and liposome was observed. The above phenomena were also supported by the fluorescence spectroscopic analysis using laurdan, resulting in the variation in the membrane properties of liposome after its mixing with Span 80 vesicle. These results suggest that the Span 80 vesicles can easily interact and hemifuse with the liposome membrane, which depend on the membrane properties of the Span 80 vesicle, "flexible" and "molecular structure" of the components. (C) 2012 Elsevier B.V. All rights reserved.