Mast cell participation during the elicitation of murine allergic contact hypersensitivity.
Mast cell participation during the elicitation of murine allergic contact hypersensitivity.
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肥大细胞参与诱发小鼠过敏性接触超敏反应。
DOI：
10.1111/1523-1747.ep12470344
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Soter,NA
中科院分区：
文献类型：
--
作者：
Kerdel,FA;Belsito,DV;Scotto-Chinnici,R;Soter,NA
为了评估肥大细胞在过敏性接触超敏反应（ACH）中的参与，用0.1%三硝基氯苯（TNCB）致敏BALB/c小鼠。攻毒前即刻和1、1.5、2.在用1%TNCB攻击后4、6、8、12、24和48 h，测量各组动物的耳厚度，采集血液用于组胺测定，并取出两个汽车用于肥大细胞的组织学评价。（14.3 ± 1.6 × 10- 2 mm;平均值± SEM），8 h（19.9 ± 1.8 × 10- 2 mm）和24 h（30.2 ± 2.9 × 10- 2 mm）。在1-4 h（超过对照水平117%）、12 h（131%）和48 h（133%）观察到血清组胺增加的三相模式。攻毒动物的组织标本检查显示，在1 - 6 h之间肥大细胞出现中度（1 +）脱颗粒，在12 h时出现广泛（2+）脱颗粒。此外，在1 - 6 h、24 h和48 h时，低颗粒肥大细胞明显。在任何时间，肥大细胞数量均无统计学显著差异。血小板和血液中的其他有形成分均未导致血液组胺水平升高。这些数据表明，肥大细胞在ACH期间以三相模式激活，因此表明肥大细胞及其产物在ACH演变中的早期和晚期作用。
In order to evaluate mast cell participation in allergic contact hypersensitivity (ACH), BALB/c mice were sensitized with 0.1% trinitrochlorobenzene (TNCB). Immediately before challenge and at 1, 1.5, 2. 4, 6, 8, 12, 24, and 48 h after challenge with 1 % TNCB, groups of animals had ear thickness measured, had blood collected for histamine determinations, and had both cars removed for histologic evaluation of mast cells.The increase in car swelling was triphasic with peak increases at 1.5 h (14.3 ± 1.6 × 10-2mm; mean ± SEM), 8 h(19.9 ± 1.8 × 10-2mm), and 24 h (30.2 ± 2.9 × 10-2mm). A triphasic pattern of increased serum histamine was noted at 1–4 h (117% over control levels), at 12 h (131 %) and at 48 h (133%). Examination of the tissue specimens from challenged animals showed modest (1 +) degranulation of mast cells between 1 and 6 h with extensive (2+) degranulation at 12 h. In addition, hypogranulated mast cells were evident between 1 and 6 h, at 24 h, and at 48 h. There were no statistically significant differences in mast cell numbers at any time. Neither platelets nor other formed elements of the blood contributed to the increased blood histamine levels. These data show that mast cells arc activated in a triphasic pattern during ACH, and thus suggest both early and late roles for the mast cell and its products in the evolution of ACH.