Covalent modified hydrophilic polymer brushes onto poly(dimethylsiloxane) microchannel surface for electrophoresis separation of amino acids
Covalent modified hydrophilic polymer brushes onto poly(dimethylsiloxane) microchannel surface for electrophoresis separation of amino acids
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DOI：
10.1016/j.chroma.2008.03.038
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发表时间：
2008-05-23
影响因子：
4.1
通讯作者：
Fan, Jing
中科院分区：
文献类型：
--
作者：
Wang, Ai-Jun;Feng, Jiu-Ju;Fan, Jing
提出了一种新的环境友好的方法，通过原位共价修饰来防止非特异性生物分子在聚二甲基硅氧烷（PDMS）表面的吸附。采用空气等离子体预处理PDMS微通道表面，并用3-氨丙基三乙氧基硅烷（APTES）进行硅烷化处理，然后在微通道表面共价接枝邻-（N-琥珀酰亚胺基）琥珀酰基-o '-甲基聚乙二醇（NSS-mPEG）。改性过程在水溶液中进行，没有任何有机溶剂。mPEG侧链表现出延伸结构，在PDMS表面形成非离子亲水聚合物刷层，可以有效阻止生物分子的吸附。该方法提高了电渗流分离的重现性和稳定性，增强了表面亲水性和峰的分辨率，减少了生物分子的吸附。与天然和硅烷化PDMS微芯片相比，改性微通道中的荧光被强烈抑制。7种氨基酸已被有效地分离，并成功地检测到涂层PDMS微芯片耦合端通道安培检测。其运行间、日间和芯片间迁移时间的相对标准偏差（RSD）均低于2.3%。此外，共价修饰的PDMS通道显示出4周的长期稳定性。这种新的涂层策略在生物分子分离中显示出良好的应用前景。(C)2008 Elsevier B. V.保留所有权利。
A new environmentally friendly method is developed for preventing nonspecific biomolecules from adsorption on poly(dimethylsiloxane) (PDMS) surface via in situ covalent modification. o-[(N-Succinimdyl)succiny]-o'-methyl-poly(ethylene glycol) (NSS-mPEG) was covalently grafted onto PDMS microchannel surface that was pretreated by air-plasma and silanized with 3-aminopropyl-triethoxysilanes (APTES). The modification processes were carried out in aqueous solution without any organic solvent. The mPEG side chains displayed extended structure and created a nonionic hydrophilic polymer brushes layer on PDMS surface, which can effectively prevent the adsorption of biomolecules. The developed method had improved reproducibility of separation and stability of electroosmotic flow (EOF), enhanced hydrophilicity of surface and peak resolution, and decreased adsorption of biomolecules. EOF in the modified microchannel was strongly suppressed, compared with those in the native and silanized PDMS microchips. Seven amino acids have been efficiently separated and successfully detected on the coated PDMS microchip coupled with end-channel amperometric detection. Relative standard deviations (RSDs) of their migration time for run-to-run, day-to-day and chip-to-chip, were all below 2.3%. Moreover, the covalent-modified PDMS channels displayed long-term stability for 4 weeks. This novel coating strategy showed promising application in biomolecules separation. (C) 2008 Elsevier B.V. All rights reserved.