Refinement and standardization of synthetic biological parts and devices
Refinement and standardization of synthetic biological parts and devices
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DOI：
10.1038/nbt1413
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发表时间：
2008-07-01
影响因子：
46.9
通讯作者：
Endy, Drew
中科院分区：
文献类型：
--
作者：
Canton, Barry;Labno, Anna;Endy, Drew
从标准可互换零件库中快速可靠地设计多组件系统的能力是现代技术的一个标志。生命系统的复杂性是否能让生物工程师开发出类似的能力，是一个迫切的研究问题。我们建议适应现有的框架，用于描述工程设备的生物对象，以（i）直接细化和使用的生物“部件”和“设备”，（ii）支持研究，使可靠的组成标准的生物部件和（iii）促进发展的抽象层次结构，简化生物工程。我们使用由此产生的框架来描述一个工程生物设备，一个基因编码的细胞间通信接收器，名为BBa_F2620。接收器的描述通过类似于工程中广泛使用的“符号”进行总结。导致BBa_F2620编码的细化和表征过程可以作为产生许多标准化遗传编码对象的起始模板。
The ability to quickly and reliably engineer many-component systems from libraries of standard interchangeable parts is one hallmark of modern technologies. Whether the apparent complexity of living systems will permit biological engineers to develop similar capabilities is a pressing research question. We propose to adapt existing frameworks for describing engineered devices to biological objects in order to (i) direct the refinement and use of biological 'parts' and 'devices', (ii) support research on enabling reliable composition of standard biological parts and (iii) facilitate the development of abstraction hierarchies that simplify biological engineering. We use the resulting framework to describe one engineered biological device, a genetically encoded cell-cell communication receiver named BBa_F2620. The description of the receiver is summarized via a 'datasheet' similar to those widely used in engineering. The process of refinement and characterization leading to the BBa_F2620 datasheet may serve as a starting template for producing many standardized genetically encoded objects.