Cross-Linking Enhances Deposition of Human Endothelial Progenitor Cells in the Rat Heart After Intracoronary Transplantation
Cross-Linking Enhances Deposition of Human Endothelial Progenitor Cells in the Rat Heart After Intracoronary Transplantation
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DOI：
10.3727/096368909x474834
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发表时间：
2010-01-01
影响因子：
3.3
通讯作者：
Schrader, Juergen
中科院分区：
文献类型：
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作者：
Burghoff, Sandra;Ding, Zhaoping;Schrader, Juergen
移植人内皮祖细胞（hEPCs）可以改善心肌梗死后的血管形成和左室功能。本研究的范围是探索，是否交联的内皮祖细胞可能会增加沉积的细胞在大鼠心脏后，临床样，冠状动脉内移植。为此，在控制条件下和缺血/再灌注后，通过微创技术将In-III-oxinate标记的hEPCs注入免疫抑制的Wistar大鼠的冠状动脉中。在第二组实验中，在移植前用植物血凝素处理hEPCs以产生小细胞簇。1小时和48小时后的连续三维HiSPECT图像显示，当hEPCs交联时，细胞沉积显著增加。因此，hEPCs的交联可以提供一种有前途的方法，以增加捕获的细胞的数量，也在临床环境中。
Transplantation of human endothelial progenitor cells (hEPCs) may improve vascularization and left ventricular function after myocardial infarction. The scope of this study was to explore, whether cross-linking of EPCs may enhance the deposition of cells in the rat heart after clinical-like, intracoronary transplantation. To this end, In-III-oxinate-labeled hEPCs were infused by a minimally invasive technique into the coronary arteries of immunosuppressed Wistar rats under control conditions and after ischemia/reperfusion. In a second set of experiments hEPCs were treated with phytohemagglutinin to create small cell clusters prior to transplantation. Continous three-dimensional HiSPECT images for 1 h and after 48 h revealed that cell deposition was significantly higher when hEPCs were cross-linked. Therefore, cross-linking of hEPCs may provide a promising approach to enhance the number of trapped cells also in a clinical setting.