Isolation of melanoma tumor-initiating cells from surgical tissues.
Isolation of melanoma tumor-initiating cells from surgical tissues.
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DOI：
10.1007/978-1-62703-227-8_16
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Boiko, Alexander D
中科院分区：
文献类型：
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作者：
Boiko, Alexander D
已经提出了一种新的癌症进展模型，该模型预测在不均匀的块状肿瘤中存在自我更新的、对化疗和放射治疗具有抵抗力并在其进展或复发过程中维持肿瘤生长的肿瘤干细胞(Ailles and Weissman，Curr Opin Biotechnol 18：460-466,2007；Chan等，Proc Natl Acad Sci U S A 106：14016-14021,2009年；D‘Angelo和Wicha，Prog Mol Biol Transl Sci 95：113-158,2010；O’Brien，Semin Radiat Onol 19：71-77,2009；Park等人，Mol Ther 17：219-230,2009)。使用最先进的细胞纯化和移植方法，我们的实验室和另一项独立研究从外科人类标本中鉴定出黑色素瘤干细胞为CD271(NFGR/p75)+细胞(Boiko等人，自然杂志466：133-137,2010；Civenni等人，癌症资源71：3098-3109,2011)。在这里，我们非常详细地描述了一种从新切除的黑色素瘤中分离肿瘤启动细胞的方法。
A new model of cancer progression has been put forward that predicts existence of tumor stem cells (TSCs) in the heterogeneous bulk tumor mass that self-renew, are resistant to chemo- and radiotherapies, and sustain tumor growth during the course of its progression or relapse ( Ailles and Weissman, Curr Opin Biotechnol 18:460–466, 2007; Chan et al., Proc Natl Acad Sci U S A 106:14016–14021, 2009; D’Angelo and Wicha, Prog Mol Biol Transl Sci 95:113–158, 2010; O’Brien, Semin Radiat Oncol 19:71–77, 2009; Park et al., Mol Ther 17:219–230, 2009). Using most advanced methods of cell purification and transplantation, our laboratory and another independent study identified melanoma stem cells as CD271(NFGR/p75)+ cells from surgical human specimens (Boiko et al., Nature 466:133–137, 2010; Civenni et al., Cancer Res 71:3098–3109, 2011). Here we describe in great detail an approach for isolating tumor-initiating cells from freshly resected melanomas.