A BIOFILM USED AS ULTRAVIOLET‐DOSIMETER
A BIOFILM USED AS ULTRAVIOLET‐DOSIMETER
复制标题
用作紫外线剂量计的生物膜
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
H. Bücker
中科院分区：
文献类型：
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作者：
L. Quintern;G. Horneck;U. Eschweiler;H. Bücker
摘要-已经开发了一种用于常规测量的紫外线剂量计，其主要根据光谱的各种成分对微生物的破坏作用进行加权。为此，构建了生物膜，其包含固定在透明聚酯塑料片上的枯草芽孢杆菌（野生型或DNA修复缺陷菌株）的干燥孢子。照射后，将生物膜在生长培养基中孵育。固定化微生物在孢子萌发和几次细胞分裂后合成的蛋白质被染色并通过光度法测定，给出生物活性的量度。根据校准曲线确定“生物有效剂量”。它反映了与产生相同效应的校准源的剂量相当的剂量。
Abstract— A UV‐dosimeter has been developed for routine measurements which mainly weights the various components of the spectrum in relation to their damaging effects on a microorganism. For this purpose a biofilm was constructed, comprising dried spores of Bacillus subtilis (wild‐type or DNA repair defective strain), immobilized on transparent polyester plastic sheets. After irradiation, the biofilm was incubated in a growth medium. The proteins, synthesized by the immobilized microorganisms after spore germination and several cell divisions, were stained and determined by photometry, giving a measure of the biological activity. The ”biologically effective dose“ was determined from a calibration curve. It reflects the dose equivalent to that of the calibration source producing the same effect.