Development of microsatellite markers from an ectomycorrhizal fungus, Tricholoma matsutake, by an ISSR-suppression-PCR method
Development of microsatellite markers from an ectomycorrhizal fungus, Tricholoma matsutake, by an ISSR-suppression-PCR method
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DOI：
10.1007/s00572-002-0193-6
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发表时间：
2003-03-01
期刊：
影响因子：
3.9
通讯作者：
Yamada, A
中科院分区：
文献类型：
--
作者：
Lian, CL;Hogetsu, T;Yamada, A
应用一种用于植物微卫星标记的简单重复序列间（ISSR）抑制PCR技术，对外生菌根真菌松茸进行了研究。共开发了6个多态性SSR标记。6个多态性SSR标记均为单位点共显性。由这6个单位点标记产生的等位基因的范围从2个到9个每个位点和预期的杂合性计算值从0.098到0.803。结果表明，ISSR-suppression-PCR技术是一种有效的、适用于外生菌根真菌微卫星标记开发的方法。此外，6个微卫星位点没有扩增任何其他外生菌根物种的DNA调查，除了口蘑（瑞典松茸）和黄栗口蘑，表明群体遗传学和繁殖的松茸可以在本研究中开发的SSR标记进行调查。
An inter-simple sequence repeat (ISSR)-suppression-PCR technique established to develop microsatellite markers of plant species was applied to an ectomycorrhizal fungus, Tricholoma matsutake. Six polymorphic SSR markers were developed. All six polymorphic SSR markers were single-locused and co-dominant. Alleles produced by these six single-locused markers ranged from two to nine per locus and the expected heterozygosities were calculated as values from 0.098 to 0.803. The results indicated that the ISSR-suppression-PCR technique was effective and applicable to the development of microsatellite markers from ectomycorrhizal fungi. Furthermore, the six microsatellite loci did not amplify DNA from any other ectomycorrhizal species investigated, except for Tricholoma nauseosum (Swedish matsutake) and Tricholoma fulvocastaneum, suggesting that population genetics and reproduction of T matsutake could be investigated by the SSR markers developed in the present study.