The use of monoclonal antibodies to quantify the levels of sialoglycoproteins α and δ and variant sialoglycoproteins in human erythrocyte membranes
The use of monoclonal antibodies to quantify the levels of sialoglycoproteins α and δ and variant sialoglycoproteins in human erythrocyte membranes
复制标题
使用单克隆抗体定量人红细胞膜中唾液酸糖蛋白 α 和 δ 以及变异唾液酸糖蛋白的水平
DOI：
10.1042/bj2330093
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发表时间：
1986
影响因子：
4.1
通讯作者：
D. Anstee
中科院分区：
文献类型：
--
作者：
A. Merry;C. Hodson;E. Thomson;G. Mallinson;D. Anstee
通过使用放射性碘标记的单克隆抗体，我们估计每个正常人红细胞中约有60万个唾液酸糖蛋白α（同义血型糖蛋白A）拷贝和8万个唾液酸糖蛋白δ（同义血型糖蛋白B）拷贝。仅表达一种α基因产物的红细胞含有约300 000个α拷贝/细胞。研究了含有α-δ杂交分子的两种红细胞类型。那些杂合表达（alpha-delta）Mi.V基因的细胞含有约100 000个alpha-delta拷贝/细胞，而那些杂合表达En（UK）基因的细胞含有约80 000个alpha-delta拷贝/细胞。还研究了含有δ-α杂交分子的红细胞类型。在具有（δ-α）Dantu基因杂合表达的细胞（供体M. P.）中测量到约200 000个（δ-α）Dantu拷贝，而在供体J. O.的红细胞上发现了约315000个推定的（δ-α）丹徒杂种拷贝。[其也具有推定的（δ-α）Dantu基因的杂合表达]。供体M. P.的红细胞具有正常的α水平，而供体J.O.的红细胞具有正常的α水平。只有正常水平的一半。作者认为，供体J. O.是α-δ-α组成[（α-δ-α）Dantu]而不是δ-α，是由类似于产生帕奇曼血红蛋白的双重交换产生的。
By using radioiodinated monoclonal antibodies we have estimated that there are about 600 000 copies of sialoglycoprotein alpha (synonym glycophorin A) and 80 000 copies of sialoglycoprotein delta (synonym glycophorin B) per normal human erythrocyte. Erythrocytes expressing the product of only one alpha gene contain about 300 000 copies of alpha/cell. Two erythrocyte types containing alpha-delta hybrid molecules were studied. Those with heterozygous expression of the (alpha-delta)Mi.V gene contain about 100 000 alpha-delta copies per cell, whereas those with heterozygous expression of the En(UK) gene contain about 80 000 alpha-delta copies/cell. Erythrocyte types containing delta-alpha hybrid molecules were also studied. About 200 000 copies of (delta-alpha)Dantu were measured in cells with heterozygous expression of the (delta-alpha)Dantu gene (donor M.P.), whereas about 315 000 copies of the putative (delta-alpha)Dantu hybrid were found on the erythrocytes of donor J.O. [which also have heterozygous expression of the putative (delta-alpha)Dantu gene]. The erythrocytes of donor M.P. have normal levels of alpha, whereas those of donor J.O. have only about half-normal levels. It is proposed that the hybrid sialoglycoprotein of donor J.O. is of alpha-delta-alpha composition [(alpha-delta-alpha)Dantu] rather than delta-alpha and results from a double cross-over analogous to that which gives rise to haemoglobin Parchman.