REVERSAL OF INTEGRATION AND DNA SPLICING MEDIATED BY INTEGRASE OF HUMAN-IMMUNODEFICIENCY-VIRUS
REVERSAL OF INTEGRATION AND DNA SPLICING MEDIATED BY INTEGRASE OF HUMAN-IMMUNODEFICIENCY-VIRUS
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DOI：
10.1126/science.1738845
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发表时间：
1992-02-07
期刊：
影响因子：
56.9
通讯作者：
BROWN, PO
中科院分区：
文献类型：
--
作者：
CHOW, SA;VINCENT, KA;BROWN, PO
在逆转录病毒整合中，病毒整合蛋白（整合酶）介导协同的DNA切割-连接反应，其中靶DNA被切割，并且所得的靶DNA的5'末端连接到病毒DNA的3'末端。通过一个寡核苷酸底物，模拟重组中间体形成的这种初始的切割连接反应，纯化的整合酶的人类免疫缺陷病毒被证明促进相同的反应，在相反的过程中，称为解体。一组结构相关的底物的分析表明，整合酶可以促进一系列的DNA切割连接反应。当崩解底物的病毒DNA组分为单链时，整合酶可以介导类似于RNA剪接的DNA剪接反应。
In retroviral integration, the viral integration protein (integrase) mediates a concerted DNA cleavage-ligation reaction in which the target DNA is cleaved and the resulting 5' ends of target DNA are joined to the 3' ends of viral DNA. Through an oligonucleotide substrate that mimics the recombination intermediate formed by this initial cleavage-ligation reaction, the purified integrase of human immunodeficiency virus was shown to promote the same reaction in reverse, a process called disintegration. Analysis of a set of structurally related substrates showed that integrase could promote a range of DNA cleavage-ligation reactions. When the viral DNA component of the disintegration substrate was single-stranded, integrase could mediate a DNA splicing reaction analogous to RNA splicing.