Electrospray photoionization (ESPI) liquid chromatography/mass spectrometry for the simultaneous analysis of cyclodextrin and pharmaceuticals and their binding interactions.
Electrospray photoionization (ESPI) liquid chromatography/mass spectrometry for the simultaneous analysis of cyclodextrin and pharmaceuticals and their binding interactions.
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DOI：
10.1002/rcm.3396
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发表时间：
2008-02
期刊：
影响因子：
--
通讯作者：
L. Short;J. Syage
中科院分区：
文献类型：
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作者：
L. Short;J. Syage
我们报告了使用多模式电喷雾电离/大气压光电离源（简称 ESI/APPI 或 ESPI）和液相色谱/质谱 (LC/MS) 来测量混合极性液体样品的所有成分，其中包含：(1) 低极性成分（激素、药物或甾醇）、(2) 极性成分（环糊精底物）和 (3) 结合的极性复合物。与单一 ESI 和多模式电喷雾电离/化学电离 (ESCI) 分析相比，ESPI 源具有多种优势，包括增强的结合复合物检测以及在较低溶剂流速下更好的性能。相对结合常数通过 (i) ESI 模式确定，产生相对 R(ESI-MS) 值，以及 (ii) ESI 和 APPI 模式，提供相对 K(D) 值。我们发现环糊精的低分子取代 (Ms) 值（即 Ms = 0.4）优先与测试的低极性化合物结合。这项研究旨在证明 ESPI 作为研究混合极性结合系统的附加工具的可行性，为所有溶液组分（极性和非极性）提供质量特异性数据。
We report on the use of a multimode electrospray ionization/atmospheric pressure photoionization source (ESI/APPI or ESPI for short) with liquid chromatography/mass spectrometry (LC/MS) to measure all components of a mixed-polarity liquid sample containing: (1) low-polarity component (hormone, pharmaceutical or sterol), (2) polar component (cyclodextrin substrate), and (3) bound polar complex. The ESPI source has several advantages over both single ESI and multimode electrospray ionization/chemical ionization (ESCI) analysis, including an enhanced bound-complex detection and better performance at lower solvent flow rates. Relative binding constants are determined with (i) ESI mode, resulting in relative R(ESI-MS) values, and (ii) both ESI and APPI modes, providing relative K(D) values. We find that low molecular-substitution (Ms) values of cyclodextrin, i.e., Ms = 0.4, preferentially bind to the low-polarity compounds tested. This investigation is intended to demonstrate the feasibility of ESPI as an additional tool for investigating mixed-polarity binding systems, providing mass-specific data for all solution components, both polar and non-polar.