Dietary supplementation with a specific mannan-rich yeast parietal fraction enhances the gut and skin mucosal barriers of Atlantic salmon (Salmo salar) and reduces its susceptibility to sea lice (Lepeophtheirus salmonis)
Dietary supplementation with a specific mannan-rich yeast parietal fraction enhances the gut and skin mucosal barriers of Atlantic salmon (Salmo salar) and reduces its susceptibility to sea lice (Lepeophtheirus salmonis)
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DOI：
10.1016/j.aquaculture.2020.735701
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发表时间：
2020-12-15
期刊：
影响因子：
4.5
通讯作者：
Merrifield, Daniel
中科院分区：
文献类型：
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作者：
Leclercq, Eric;Pontefract, Nicola;Merrifield, Daniel
背景：大西洋鲑鱼（Salmo salar）养殖业越来越依赖非药物干预措施来控制海虱，导致严重的皮肤粘膜紊乱和间接健康问题。基于酵母的 MOS 产品的膳食补充剂被广泛用于支持养殖物种的肠道稳态。关于它们对水生物种皮肤粘膜的影响的证据越来越多，但仍然不一致，并且在某种程度上缺乏对海虱管理的明显贡献。进行了一项基于罐的试验，以测试基于酵母的 MOS 功能化合物 (sMOS) 对皮肤粘膜层的作用及其对海虱 (Lepeophtheirus salminis) 的保护作用。结果：在膳食补充剂 6 周后，测试化合物显着增加皮肤粘液 (+46%) 和杯状细胞密度 (+25%)，同时对肠绒毛长度 (+10.9%) 和杯状细胞密度产生积极影响(+80.0%) 也被记录下来。膳食补充剂后，对急性标准桡足类挑战的敏感性降低了 16.6%，同时皮肤溶菌酶活性也较早增加，广泛用作先天免疫的指标。结论：该研究提供了功能性证据，证明膳食 sMOS 的益处超越肠道，到达皮肤粘膜。加强大西洋鲑鱼的皮肤屏障和免疫功能以及由此降低的海虱易感性有可能减少除虱干预措施的需要以及非药物干预措施对动物健康和福利的影响。
Background: Increasing reliance on non-medicinal interventions to control sea lice in the Atlantic salmon (Salmo salar) farming industry imposes a high level of skin mucosal disturbance and indirect health issues. Dietary supplementation with yeast-based MOS products is widely used to support intestinal homeostasis across farmed species. Evidence of their effect on skin mucosa is increasing in aquatic species but it remains inconsistent and someway short of a clear contribution to sea lice management. A tank-based trial was performed to test the effect of a yeast-based MOS functional compound (sMOS) on the skin mucosal layer and its protective effects against sea lice (Lepeophtheirus salmonis).Results: The test compound significantly increased skin mucus (+46%) and goblet cell density (+25%) after 6 weeks of dietary supplementation when positive effects on intestinal villi-length (+10.9%) and goblet cell density (+80.0%) were also documented. Following dietary supplementation, a 16.6% reduction in susceptibility to an acute standard copepodid challenge was measured alongside an earlier increase in skin lysozyme activity widely used as an index of innate immunity.Conclusion: The study provides functional evidence that the benefits of dietary sMOS reach beyond the intestine to the skin mucosa. Bolstering of the Atlantic salmon skin barrier and immune functions and the resulting lower susceptibility to sea lice has the potential to reduce the need for delousing interventions and the impact of non-medicinal interventions on the animal's health and welfare.