Crosstalk and differential response to abiotic and biotic stressors reflected at the transcriptional level of effector genes from secondary metabolism
Crosstalk and differential response to abiotic and biotic stressors reflected at the transcriptional level of effector genes from secondary metabolism
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DOI：
10.1007/s11103-004-0274-3
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发表时间：
2004-04-01
影响因子：
5.1
通讯作者：
Schäffner, AR
中科院分区：
文献类型：
--
作者：
Glombitza, S;Dubuis, PH;Schäffner, AR
植物次生代谢对不利的非生物和生物信号有重要的防御作用。为了研究应激诱导的潜在效应基因家族的转录改变，我们建立了一个包含基因特异性探针的DNA阵列（MetArray）。它包含109个二级产物糖基转移酶、63个谷胱甘肽利用酶、62个细胞色素P450单加氧酶和26个ABC转运蛋白的完整基因组。在非胁迫植物的不同器官和芽部中监测了它们对除草剂、UV-B辐射、内源胁迫激素和病原体感染的响应。基于这些效应基因家族转录的主成分分析定义了不同的应答和串扰。对两种磺脲类除草剂水杨酸酯和一株无毒丁香假单胞菌pv进行组合反应，分离出茉莉酸甲酯和乙烯处理。番茄。对除草剂溴苯醚和UV-B辐射的反应不同。此外，这些分析明确了个体效应基因，表明它们在这些应激反应中的作用。一小群基因在区分对两种除草剂的反应方面具有诊断性。有趣的是，丁香假单胞菌诱导的一部分基因对施加的应激激素没有反应。综合诱导效应基因的小群体表明了共同的防御策略。此外，这些效应基因家族分支中的同源成员在两个器官中或在应激反应中表现出不同的表达模式，这表明它们具有非冗余功能。
Plant secondary metabolism significantly contributes to defensive measures against adverse abiotic and biotic cues. To investigate stress-induced, transcriptional alterations of underlying effector gene families, which encode enzymes acting consecutively in secondary metabolism and defense reactions, a DNA array (MetArray) harboring gene-specific probes was established. It comprised complete sets of genes encoding 109 secondary product glycosyltransferases and 63 glutathione-utilizing enzymes along with 62 cytochrome P450 monooxygenases and 26 ABC transporters. Their transcriptome was monitored in different organs of unstressed plants and in shoots in response to herbicides, UV-B radiation, endogenous stress hormones, and pathogen infection. A principal component analysis based on the transcription of these effector gene families defined distinct responses and crosstalk. Methyl jasmonate and ethylene treatments were separated from a group combining reactions towards two sulfonylurea herbicides, salicylate and an avirulent strain of Pseudomonas syringae pv. tomato. The responses to the herbicide bromoxynil and UV-B radiation were distinct from both groups. In addition, these analyses pinpointed individual effector genes indicating their role in these stress responses. A small group of genes was diagnostic in differentiating the response to two herbicide classes used. Interestingly, a subset of genes induced by P. syringae was not responsive to the applied stress hormones. Small groups of comprehensively induced effector genes indicate common defense strategies. Furthermore, homologous members within branches of these effector gene families displayed differential expression patterns either in both organs or during stress responses arguing for their non-redundant functions.