Genetic characterization of hepatitis E virus from wild boar in China
Genetic characterization of hepatitis E virus from wild boar in China
复制标题
中国野猪戊型肝炎病毒的遗传特征
DOI：
10.1111/tbed.14633
复制
发表时间：
2022-06-30
影响因子：
4.3
通讯作者：
Tu, Zhongzhong
中科院分区：
文献类型：
--
作者：
Gong, Wenjie;Du, Haiying;Tu, Zhongzhong
戊型肝炎病毒(HEV)是引起戊型肝炎(HE)的主要病原，分为4种主要的基因型(1-4)，其中野猪是3、4型的主要天然宿主。然而，对中国野猪中HEV感染的流行情况知之甚少。本研究采用逆转录-套式聚合酶链式反应和逆转录-定量聚合酶链式反应检测了2018年至2020年从中国24个地区采集的331头散养野猪的组织标本中的HEVRNA，并对阳性样本的部分ORF2基因或全基因组进行了测序。并对216份野猪血清标本进行了HEV抗体检测。结果在分布于8个地区的331份野猪肝脏标本中检出9份(2.72%)HEV RNA。ORF2部分序列的遗传和进化分析表明，本研究中鉴定的HEV毒株核苷酸序列同源性为83.9%~100%，分别属于4d亚型(n=6)、4g亚型(n=2)和4h亚型(n=1)，7株野猪HEV的全基因组序列具有相似的系统发育关系。此外，肝脏中的HEV病毒载量高于其他组织和血液中的载量。此外，216份野猪血清中有61份(28.2%)抗-HEV抗体阳性。据了解，这是首次报道中国地区散养野猪中HEV感染的流行病学情况，所获得的数据对HEV的防控具有一定的参考价值。
Hepatitis E virus (HEV), the causative agent of hepatitis E (HE), is classified into four major genotypes (1-4), with wild boar being the main natural reservoir for genotypes 3 and 4. However, little is known about the prevalence of HEV infection in wild boars in China. In this study, RT-nested PCR and RT-quantitative PCR were used to detect the HEV RNA in tissue samples taken from 331 free-ranging wild boars collected between 2018 and 2020 from 24 regions across China, and the partial ORF2 genes or complete genomes of the positive samples were sequenced. Furthermore, antibodies against HEV in 216 serum samples from wild boars were tested by ELISA. As a result, HEV RNA was detected in nine out of 331 liver samples of wild boars (2.72%), which were distributed in eight regions. Genetic and evolutionary analysis of partial ORF2 sequences indicated that the HEV strains identified in this study share 83.9%-100% nucleotide sequence identity and belong to subtypes 4d (n = 6), 4g (n = 2), and 4h (n = 1), and similar phylogeny was obtained using the complete genome sequences of seven wild boar HEV strains. Additionally, the HEV viral loads were higher in the liver than in other tissues and blood. Moreover, 61 out of 216 sera (28.2%) from wild boars tested positive for anti-HEV antibodies. To our knowledge, this is the first study to report the epidemiological situations of HEV infections in free-ranging wild boars in China, and the obtained data are valuable for prevention and control of HE.