MULTIPOTENT PRECURSORS CAN GIVE RISE TO ALL MAJOR CELL-TYPES OF THE FROG RETINA
MULTIPOTENT PRECURSORS CAN GIVE RISE TO ALL MAJOR CELL-TYPES OF THE FROG RETINA
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DOI：
10.1126/science.2449732
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发表时间：
1988-03-04
期刊：
影响因子：
56.9
通讯作者：
FRASER, SE
中科院分区：
文献类型：
--
作者：
WETTS, R;FRASER, SE
进行了前瞻性谱系分析，以确定不同的细胞类型，可以形成的个别前体细胞的发展青蛙视网膜。将荧光葡聚糖离子电渗注入胚胎视泡的单细胞中。在胚胎进一步发育后，在幼虫视网膜的所有三层中都观察到标记的后代。此外，不同的克隆由所有主要细胞类型的各种组合组成，包括神经胶质Muller细胞。因此，单个视泡细胞具有形成任何类型的视网膜细胞的潜力，这表明指定视网膜细胞分化途径的相互作用必须在发育后期发生。
A prospective lineage analysis was performed to determine the variety of cell types that could be formed by individual precursor cells of the developing frog retina. Fluorescent dextran was iontophoretically injected into single cells of the embryonic optic vesicle. After further development of the embryo, labeled descendants were observed in all three layers of the larval retinas. Furthermore, different clones were composed of various combinations of all major cell types, including the glial Muller cells. Hence, single optic vesicle cells have the potential to form any type of retinal cell, suggesting that the interactions that specify the differentiation pathway of retinal cells must occur late in development.