BLOOD-FLOW OF THE URINARY-BLADDER - EFFECTS OF OUTLET OBSTRUCTION AND CORRELATION WITH BIOENERGETIC METABOLISM
BLOOD-FLOW OF THE URINARY-BLADDER - EFFECTS OF OUTLET OBSTRUCTION AND CORRELATION WITH BIOENERGETIC METABOLISM
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DOI：
10.1002/nau.1930140309
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发表时间：
1995-01-01
影响因子：
2
通讯作者：
CHANG, LS
中科院分区：
文献类型：
--
作者：
LIN, ATL;CHEN, MT;CHANG, LS
本实验研究了出口梗阻对兔膀胱血流和高能磷酸盐含量的影响。每只雄性新西兰大白兔(n=7)在膀胱颈上放置直径7 mm的硅环，造成轻度膀胱出口梗阻。在诱导梗阻之前和之后，以及2周后，排空膀胱，并使用激光多普勒血流仪(LASERFLO BPM(2)，Vasamedics Inc.，St.Paul，明尼苏达州)测量局部血流量。测量了每个膀胱的六个不同区域，并计算了每只兔子的平均血流量。然后处死动物，切除膀胱，用高效液相色谱法测定组织中高能磷酸盐的含量。6只正常雄性新西兰大白兔作为对照组。结果如下：(1)术前所有动物膀胱血流量相似(16.3ml/min/100g)，放置硅环不影响膀胱颈血液灌注量，诱导出口梗阻2周后，膀胱血流量明显减少(4.9ml/min/100g)。(2)对照组与梗阻性膀胱组NAD、AMP、ADP含量差异无统计学意义。然而，与对照组相比，梗阻膀胱的磷酸肌酸(12.0比21.9nmol/mg蛋白质)和三磷酸腺苷(4.0nmol/mg蛋白质比6.1nmol/mg蛋白质)含量显著减少。血流减少可能是出口梗阻后膀胱能量代谢紊乱和功能损害的重要因素。(C)1995年Wiley-Liss公司
This study investigated the effects of outlet obstruction on blood flow and high energy phosphates content in the rabbit urinary bladder. Mild bladder outlet obstruction was induced by placing a silicon ring (diameter 7 mm) around the bladder neck of each male New Zealand White rabbit (n = 7). Before and immediately after inducing obstruction, and 2 weeks later, the bladders were emptied and regional blood flow measured using laser Doppler flowmetry (LASERFLO BPM(2), Vasamedics Inc., St. Paul, Minnesota). Six different areas of each bladder were measured, and the average blood flow calculated for each rabbit. Then, the animals were sacrificed, the bladder excised, and the tissue content of high energy phosphates determined by high performance liquid chromatography (HPLC). Six normal male New Zealand White rabbits served as controls. The results can be summarized as follows: (1) Before surgery, bladder blood flow was similar in all animals (16.3 ml/min/100 g); positioning the silicon ring around the bladder neck did not affect blood perfusion, two weeks after the induction of outlet obstruction, bladder blood flow was significantly decreased (4.9 ml/min/100 g). (2) There was no significant difference between control and obstructed bladders in NAD, AMP, or ADP content. However, the obstructed bladders contained significantly less phosphocreatine (12.0 vs 21.9 nmol/mg protein) and ATP (4.0 vs. 6.1 nmol/mg protein) than control bladders.In summary, this study showed that urinary bladder blood flow was reduced by outlet obstruction, and the reduction in blood flow was associated with decreased tissue high energy phosphates content. Decreased blood flow might be an important factor in the disarranged energy metabolism and functional impairment exhibited by the urinary bladder following outlet obstruction. (C) 1995 Wiley-Liss, Inc.