Analysis of syn- and anti-1,2-dihydroxy-3,4-epoxy-1,2,3,4-tetrahydro-5-methylchrysene -deoxyribonucleoside adducts by boronate chromatography.
Analysis of syn- and anti-1,2-dihydroxy-3,4-epoxy-1,2,3,4-tetrahydro-5-methylchrysene -deoxyribonucleoside adducts by boronate chromatography.
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通过硼酸盐色谱法分析顺式和反式 1,2-二羟基-3,4-环氧-1,2,3,4-四氢-5-甲基-脱氧核糖核苷加合物。
DOI：
10.1016/0304-3835(85)90012-6
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发表时间：
1985
期刊：
影响因子：
9.7
通讯作者：
Harvey,RG
中科院分区：
文献类型：
--
作者：
Melikian,AA;Hecht,SS;Hoffmann,D;Pataki,J;Harvey,RG
The Servacel DHB (m-dihydroxyborylphenylaminoethyl cellulose) chromatographic procedure developed by Sawiki et al. (Cancer Res., 43, 3212–3218, 1983) for analysis of 7,12-dimethylbenz[a]anthracene (DMBA)-DNA adducts was applied to analyzesyn- andanti-1,2-dihydroxy-3,4-epoxy-1,2,3,4-tetrahydro-5-methylchrysene (DE-I)-deoxyribonucleoside adducts. Identical elution conditions to whose developed for the DMBA adducts were employed. While the results were similar to those obtained in the DMBA system, some of theanti-DE-I-deoxyribonucleoside adducts eluted with the buffer system used for elution ofsyn-adducts. Complete resolution of theanti- andsyn-adducts was obtained when modified elution conditions as developed by Pruess-Schwartz et al. (Cancer Res., 44, 4104–4110, 1984) for analysis ofsyn- andanti-7α-8β-dihydroxy-9β, 10β-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene—DNA adducts were applied. Based on this chromatographic procedure about 15% of the DE-I deoxyribonucleoside adducts, formed in mouse skin DNA upon treatment with 5-[3H]methylchrysene (MeC), originated fromsyn-DE-I.
