Long-term warming rather than grazing significantly changed total and active soil procaryotic community structures
Long-term warming rather than grazing significantly changed total and active soil procaryotic community structures
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长期变暖而不是放牧显着改变了土壤原核生物群落结构的总量和活性
DOI：
10.1016/j.geoderma.2017.12.005
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发表时间：
2018-04-15
期刊：
影响因子：
6.1
通讯作者：
Cui, Xiaoyong
中科院分区：
文献类型：
--
作者：
Che, Rongxiao;Deng, Yongcui;Cui, Xiaoyong
在了解气候变暖和放牧对土壤微生物及其活性对应物的影响方面，特别是在对全球变暖和人类活动极其敏感的青藏高原，知识非常缺乏。通过为期6年的田间试验，研究了不对称升温和适度放牧对西藏嵩草高寒草甸土壤微生物总量和活性的影响。利用实时荧光定量PCR技术测定了土壤细菌丰度和16S rDNA转录活性。利用MiSeq测序技术分别对16S rDNA和rRNA序列进行分析，研究了土壤原核生物群落结构。结果表明，与放牧相比，土壤原核生物群落对气候变暖的反应更为敏感。变暖显着降低土壤微生物呼吸速率，16S rDNA转录活性，和总原核生物群落结构的分散，但显着增加活性原核生物的α多样性。变暖也显着增加了贫营养微生物的相对丰度，而减少共养谱系的比例。从总的原核生物群落结构预测的功能配置文件仍然不受变暖的影响。然而，基于rRNA的预测表明，DNA复制，基因表达，信号转导和蛋白质降解显着抑制变暖。放牧仅显著降低了16S rDNA转录和总原核丰富度。总的来说，这些研究结果表明，变暖可以将土壤原核生物群落转变为更贫营养和更不活跃的状态，突出了调查活性微生物的重要性，以提高我们对气候变化和人类活动的生态系统反馈的理解。
There is a paucity of knowledge in understanding the effects of warming and grazing on soil microbes and their active counterparts, especially on the Tibetan Plateau which is extremely sensitive to global warming and human activities. A six-year field experiment was conducted to investigate the effects of asymmetric warming and moderate grazing on total and active soil microbes in a Tibetan Kobresia alpine meadow. Soil bacterial abundance and 16S rDNA transcriptional activity were determined using real-time PCR. Total and active soil procaryotic community structures were analyzed through MiSeq sequencing based on 16S rDNA and rRNA, respectively. The results showed that the soil procaryotic community was more sensitive to the warming than the grazing. The warming significantly decreased soil microbial respiration rates, 16S rDNA transcription activity, and dispersion of total procaryotic community structures, but significantly increased the alpha diversity of active procaryotes. Warming also significantly increased the relative abundance of oligotrophic microbes, whereas decreasing the copiotrophic lineage proportions. The functional profiles predicted from the total procaryotic community structures remained unaffected by warming. However, the rRNA-based predictions suggested that DNA replication, gene expression, signal transduction, and protein degradation were significantly suppressed under the warming. The grazing only significantly decreased the 16S rDNA transcription and total procaryotic richness. Overall, these findings suggest that warming can shift soil procaryotic community to a more oligotrophic and less active status, highlighting the importance of investigating active microbes to improve our understanding of ecosystem feedbacks to climate change and human activities.