THE EPSTEIN-BARR-VIRUS NUCLEAR ANTIGEN-2 TRANSACTIVATOR IS DIRECTED TO RESPONSE ELEMENTS BY THE J-KAPPA RECOMBINATION SIGNAL BINDING-PROTEIN
THE EPSTEIN-BARR-VIRUS NUCLEAR ANTIGEN-2 TRANSACTIVATOR IS DIRECTED TO RESPONSE ELEMENTS BY THE J-KAPPA RECOMBINATION SIGNAL BINDING-PROTEIN
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DOI：
10.1073/pnas.91.16.7568
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发表时间：
1994-08-02
影响因子：
11.1
通讯作者：
KIEFF, E
中科院分区：
文献类型：
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作者：
GROSSMAN, SR;JOHANNSEN, E;KIEFF, E
EB病毒核抗原2（EBNA-2）在原发性B淋巴细胞生长转化中起重要作用。EBNA-2是一种酸性转录反式激活因子，通过与识别双链序列MNYYGTGGGAA的因子相互作用而被带到病毒和细胞EBNA-2应答元件，其中M是A或C，N是任何核苷酸，Y是嘧啶。一个63-kDa的蛋白质，识别这种DNA序列，现在已经被纯化的S-琼脂糖和寡核苷酸亲和层析。p63肽序列与人J κ免疫球蛋白重组信号结合蛋白的预测氨基酸序列相同。纯化的或重组的体外翻译的J κ与MNYYGTGGGAA EBNA-2应答元件序列结合并与EBNA-2相互作用。令人惊讶的是，J κ不与J（κ）1七聚体重组信号序列（CACTGTG）结合，并且其先前被鉴定为七聚体结合蛋白最有可能是由于向天然七聚体添加BamHI限制性位点，从而产生近EBNA-2应答元件共有序列（CACTGTGGGAT）。
Epstein-Barr virus nuclear antigen 2 (EBNA-2) plays an essential role in primary B-lymphocyte growth transformation. EBNA-2 is an acidic transcriptional transactivator that is brought to virus and cell EBNA-2 response elements by interaction with a factor that recognizes the double-stranded sequence MNYYGTGGGAA, where M is A or C, N is any nucleotide, and Y is a pyrimidine. A 63-kDa protein that recognizes this DNA sequence has now been purified by S-Sepharose and oligonucleotide affinity chromatography. p63 peptide sequence is identical to the predicted amino acid sequence for the human J kappa immunoglobulin recombination signal binding protein. Purified or recombinant in vitro-translated J kappa binds to the MNYYGTGGGAA EBNA-2 response element sequence and interacts with EBNA-2. Surprisingly, J kappa does not bind to the J(kappa)1 heptamer recombination signal sequence (CACTGTG), and its prior identification as a heptamer binding protein was most likely due to the addition of a BamHI restriction site to the native heptamer creating a near EBNA-2 response element consensus (CACTGTGGGAT).