Marinobacter Dominates the Bacterial Community of the Ostreococcus tauri Phycosphere in Culture.
Marinobacter Dominates the Bacterial Community of the Ostreococcus tauri Phycosphere in Culture.
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DOI：
10.3389/fmicb.2016.01414
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发表时间：
2016
影响因子：
5.2
通讯作者：
Sanchez-Ferandin S
中科院分区：
文献类型：
--
作者：
Lupette J;Lami R;Krasovec M;Grimsley N;Moreau H;Piganeau G;Sanchez-Ferandin S
微藻-细菌相互作用在海洋环境中很常见，在实验室培养的硅藻中也很常见。这些相互作用也对海洋中的细菌和藻类多样性产生了强烈影响。Mamiellophyceae（绿藻门）的小型绿色真核藻类是普遍存在的，并且一些种类，如Ostreococcus spp.，在地中海沿岸泻湖中尤为重要，在公海浮游植物大量繁殖期间被视为优势物种。尽管如此，对于可能促进或阻碍O.金牛座的成长我们用rDNA 16 S序列表明，O. tauri RCC 4221实验室培养物中主要是来自海蛞蝓属的γ-变形菌，与O. tauri RCC 4221、所用的光周期或所测试的营养条件（限制在氮或磷中）。发现了几种Marinalgicola菌株，它们都与分类学上不同的生物，特别是甲藻和颗石藻密切相关。这些序列与M. adhaerens，M.通常与裸藻有关的水生物和细菌。据我们所知，这是第一次发现不同的海蛞蝓菌株与文化中的绿色蛞蝓相关。
Microalgal–bacterial interactions are commonly found in marine environments and are well known in diatom cultures maintained in laboratory. These interactions also exert strong effects on bacterial and algal diversity in the oceans. Small green eukaryote algae of the class Mamiellophyceae (Chlorophyta) are ubiquitous and some species, such as Ostreococcus spp., are particularly important in Mediterranean coastal lagoons, and are observed as dominant species during phytoplankton blooms in open sea. Despite this, little is known about the diversity of bacteria that might facilitate or hinder O. tauri growth. We show, using rDNA 16S sequences, that the bacterial community found in O. tauri RCC4221 laboratory cultures is dominated by γ-proteobacteria from the Marinobacter genus, regardless of the growth phase of O. tauri RCC4221, the photoperiod used, or the nutrient conditions (limited in nitrogen or phosphorous) tested. Several strains of Marinobacter algicola were detected, all closely related to strains found in association with taxonomically distinct organisms, particularly with dinoflagellates and coccolithophorids. These sequences were more distantly related to M. adhaerens, M. aquaeoli and bacteria usually associated to euglenoids. This is the first time, to our knowledge, that distinct Marinobacter strains have been found to be associated with a green alga in culture.