Gamma interferon is not essential in host defense against disseminated candidiasis in mice.
Gamma interferon is not essential in host defense against disseminated candidiasis in mice.
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γ干扰素对于宿主防御小鼠播散性念珠菌病并不是必需的。
DOI：
10.1128/iai.65.5.1748-1753.1997
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发表时间：
1997
影响因子：
3.1
通讯作者：
Cutler,JE
中科院分区：
文献类型：
--
作者：
Qian,Q;Cutler,JE
体外研究表明，干扰素-γ在宿主防御播散性念珠菌病中发挥了作用，但体内研究尚未得出结论。我们利用纯合子干扰素-γ基因敲除(GKO)小鼠来确定细胞因子是否在宿主防御这种疾病中是必不可少的。用针对正常或突变的干扰素-γ基因的特异性引物进行聚合酶链式反应，检测小鼠的基因分型。用酶联免疫吸附试验证实了小鼠的GKO状态，结果表明，在刀豆蛋白A刺激下，小鼠的脾细胞没有产生可检测到的干扰素-γ。为了测试GKO小鼠对念珠菌病的敏感性，动物被静脉感染(iv.或灌胃(i.g.)白色念珠菌。静脉感染GKO小鼠。与野生型(WT)小鼠一样存活，肝、脾或肾脏中的念珠菌CFU计数与WT小鼠相比没有差异。当动物在感染后3小时或10天或21天注射念珠菌时，无论是GKO小鼠还是WT小鼠，都没有发现念珠菌扩散到肺、肝、脾或肾。从胃或肠道中回收的念珠菌CFU计数在GKO和WT小鼠之间没有差异。对真菌所在的胃心-心房皱折的组织学检查表明，GKO小鼠没有证据表明比WT小鼠有更多的组织损伤或真菌侵袭。最后，两种类型的小鼠的空肠都没有显示出组织损伤或真菌入侵的证据。这些研究表明，在小鼠模型中，干扰素-γ在抵抗白色念珠菌的宿主防御中并不是必不可少的，这些白色念珠菌起源于粘膜部位或直接进入血液。
In vitro studies have suggested a role for interferon gamma (IFN-gamma) in host defense against disseminated candidiasis, but in vivo studies are inconclusive. We utilized homozygous IFN-gamma knockout (GKO) mice to determine if the cytokine is essential in host defense against this disease. Genotypes of mice were determined by PCR with specific primers for the normal or disrupted IFN-gamma gene. The GKO status of the mice was confirmed by an enzyme-linked immunosorbent assay, which showed no detectable IFN-gamma produced by their splenocytes stimulated by concanavalin A. To test the susceptibility of GKO mice to candidiasis, the animals were infected either intravenously (i.v.) or intragastrically (i.g.) with Candida albicans. GKO mice infected i.v. survived as long as wild-type (WT) mice and showed no difference in Candida CFU counts in liver, spleen, or kidneys compared to those for WT mice. When animals were given Candida i.g., at 3 h or at 10 or 21 days after infection, there was no dissemination of Candida to the lung, liver, spleen, or kidneys for either GKO or WT mice. There was no difference in Candida CFU counts recovered from the stomach or intestines between GKO and WT mice. Histological examination of the stomach cardial-atrium fold, where the fungus was located, showed that GKO mice did not have evidence of more tissue damage or fungal invasion than WT mice. Finally, the jejunum for both types of mice showed no evidence of tissue damage or fungal invasion. These studies indicate that IFN-gamma is not essential in host defense against C. albicans that originates from a mucosal site or that is given directly into the bloodstream in a mouse model.