Human skeletal muscle tissue chip autonomous payload reveals changes in fiber type and metabolic gene expression due to spaceflight.
Human skeletal muscle tissue chip autonomous payload reveals changes in fiber type and metabolic gene expression due to spaceflight.
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人骨骼肌组织芯片自主有效载荷揭示了由于太空飞行而引起的纤维类型和代谢基因表达的变化。
DOI：
10.1038/s41526-023-00322-y
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发表时间：
2023-09-15
期刊：
影响因子：
5.1
通讯作者：
Malany, Siobhan
中科院分区：
文献类型：
--
作者：
Parafati, Maddalena;Giza, Shelby;Shenoy, Tushar S.;Mojica-Santiago, Jorge A.;Hopf, Meghan;Malany, Legrand K.;Platt, Don;Moore, Isabel;Jacobs, Zachary A.;Kuehl, Paul;Rexroat, JasHainanon;Barnett, Gentry;Schmidt, Christine E.;McLamb, William T.;Clements, Twyman;Coen, Paul M.;Malany, Siobhan
微生理学系统提供了在极端空间环境中模拟人体组织一级加速变化的机会。宇航员经历的太空飞行引起的肌肉萎缩与老年人的肌肉萎缩有着相似的生理变化，称为肌肉减少症。这些共同的属性为研究暴露于太空飞行的肌肉细胞的分子变化提供了理论基础，这些变化可能模拟肌肉减少症的潜在病理生理学。我们报告了来自年轻人和老年人肌肉活检的三维肌束的结果，这些肌束集成到自主CubeLab™中，并作为NIH/NASA资助的太空组织芯片计划的一部分乘坐SpaceX CRS-21飞往国际空间站（ISS）。全球转录组RNA-Seq分析比较了空间和地面上的肌束，揭示了与成肌细胞增殖和肌肉分化相关的共享转录物的下调。分析还显示，与地面对照相比，与暴露于空间环境的老年队列中的肌束特有的肌肉代谢相关的差异表达基因途径下调。与地面对照相比，与炎症途径相关的基因类在年轻队列培养的飞行样本中下调。我们的肌肉组织芯片平台提供了一种研究航天对肌肉细胞生物学的细胞自主影响的方法，这种影响在整个器官或生物体水平上可能无法理解，并为微重力下肌肉组织芯片实验的持续数据收集奠定了基础。我们还报告了在国际空间站上进行自主组织芯片CubeLabTM有效载荷的挑战和机遇。
Microphysiological systems provide the opportunity to model accelerated changes at the human tissue level in the extreme space environment. Spaceflight-induced muscle atrophy experienced by astronauts shares similar physiological changes to muscle wasting in older adults, known as sarcopenia. These shared attributes provide a rationale for investigating molecular changes in muscle cells exposed to spaceflight that may mimic the underlying pathophysiology of sarcopenia. We report the results from three-dimensional myobundles derived from muscle biopsies from young and older adults, integrated into an autonomous CubeLab™, and flown to the International Space Station (ISS) aboard SpaceX CRS-21 as part of the NIH/NASA funded Tissue Chips in Space program. Global transcriptomic RNA-Seq analyses comparing the myobundles in space and on the ground revealed downregulation of shared transcripts related to myoblast proliferation and muscle differentiation. The analyses also revealed downregulated differentially expressed gene pathways related to muscle metabolism unique to myobundles derived from the older cohort exposed to the space environment compared to ground controls. Gene classes related to inflammatory pathways were downregulated in flight samples cultured from the younger cohort compared to ground controls. Our muscle tissue chip platform provides an approach to studying the cell autonomous effects of spaceflight on muscle cell biology that may not be appreciated on the whole organ or organism level and sets the stage for continued data collection from muscle tissue chip experimentation in microgravity. We also report on the challenges and opportunities for conducting autonomous tissue-on-chip CubeLabTM payloads on the ISS.
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影响因子：
15.3
作者：
Hara, Mie;Yuasa, Shinsuke;Fukuda, Keiichi
通讯作者：
Fukuda, Keiichi
影响因子：
7.8
作者：
通讯作者：
--
影响因子：
3.4
作者：
De Paepe, Boel;Creus, Kim K.;De Bleecker, Jan L.
通讯作者：
De Bleecker, Jan L.
影响因子：
6.3
作者：
Ham, Daniel J.;Murphy, Kate T.;Koopman, Rene
通讯作者：
Koopman, Rene
DOI：
10.18632/aging.100358
发表时间：
2011-08
期刊：
Aging
影响因子：
--
作者：
Hall DT;Ma JF;Marco SD;Gallouzi IE
通讯作者：
Gallouzi IE