Evaluation of Coptidis Rhizoma-Euodiae Fructus couple and Zuojin products based on HPLC fingerprint chromatogram and simultaneous determination of main bioactive constituents
Evaluation of Coptidis Rhizoma-Euodiae Fructus couple and Zuojin products based on HPLC fingerprint chromatogram and simultaneous determination of main bioactive constituents
复制标题
基于HPLC指纹图谱及主要活性成分同时测定的黄连-吴茱萸对及左金产品评价
DOI：
10.3109/13880209.2013.793719
复制
发表时间：
2013-11-01
影响因子：
3.8
通讯作者：
Marriott, Philip J.
中科院分区：
文献类型：
--
作者：
Gao, Xin;Yang, Xiu-Wei;Marriott, Philip J.
背景：黄连- eudiae Fructus couple （CEC）是由黄连与eudiae Fructus以6:1的比例组成的经典中药制剂，用于治疗胃肠道疾病。生物碱是主要的生物活性成分。本研究为CEC的质量控制提供了全面的分析资料。目的：建立高效液相色谱-二极管阵列检测指纹图谱，用于鉴别CEC及其制品的化学成分特征。材料和方法：采用Gemini C-18色谱柱，水甲酸（100:0.03），乙腈为流动相，梯度洗脱。流速1.0 mL/min，检测波长250 nm。采用相似度分析和主成分分析（PCA）评价分析品的质量一致性。采用“中药色谱指纹图谱相似度评价系统”软件计算分析物的平均色谱图和相关系数。结果：指纹图谱比对确定了20个具有代表性的一般指纹峰，指纹图谱相似度均优于0.988。结果一致表明，CEC及其相关样品可以成功地分为三组。通过PCA生成的贡献图来解释样本组之间的差异，并识别出对分类有显著贡献的峰。通过定量分析验证了样品中的7种生物活性成分。讨论与结论：相似度评价色谱指纹图谱和主成分分析法结合7个化合物的定量分析，可作为该药材对的质量控制方法。
Context: Coptidis Rhizoma-Euodiae Fructus couple (CEC) is a classic traditional Chinese medicine preparation consisting of Coptidis Rhizoma and Euodiae Fructus at the ratio of 6: 1, and used to treat gastro-intestinal disorders. Alkaloids are the main bioactive component. This research provides comprehensive analysis information for the quality control of CEC.Objective: To develop a high-performance liquid chromatography-diode array detection fingerprint for chemical composition characteristics of CEC and its products.Materials and methods: The samples were separated with a Gemini C-18 column by using gradient elution with water-formic acid (100:0.03) and acetonitrile as mobile phase. Flow rate was 1.0 mL/min and detection wavelength was 250 nm. Similarity analysis and principal component analysis (PCA) were employed to evaluate quality consistencies of analytes. Mean chromatograms and correlation coefficients of analytes were calculated by the software "Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine''.Results: Fingerprint chromatogram comparison determined 20 representative general fingerprint peaks, and the fingerprint chromatogram resemblances are all better than 0.988. Consistent results were obtained to show that CEC and its related samples could be successfully divided into three groups. Contribution plots generated by PCA were performed to interpret differences among the sample groups while peaks which significantly contributed to classification were identified. Seven bioactive constituents in the samples were verified by quantitative analysis.Discussion and conclusion: The chromatographic fingerprint with similarity evaluation and PCA assay combined with quantification of seven compounds could be utilized as a quality control method for the herbal couple.