Characterization of Autographa californica Nucleopolyhedrovirus Infection in Cell Lines from Bombyx mori
Characterization of Autographa californica Nucleopolyhedrovirus Infection in Cell Lines from Bombyx mori
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家蚕细胞系中苜蓿银纹夜蛾核多角体病毒感染的特征
DOI：
10.11416/jibs2001.70.49
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发表时间：
2001
影响因子：
--
通讯作者：
Michihiro Kobayashi
中科院分区：
文献类型：
--
作者：
M. Ikeda;Yasuhiro Katou;Yasufumi Yamada;S. Chaeychomsri;Michihiro Kobayashi
对家蚕细胞进行了细胞病理学、细胞活力和代谢活性、病毒DNA复制、病毒多肽合成和多角体蛋白基因表达等方面的研究，以确定家蚕细胞感染链球绦虫核型多角体病毒(AcMNPV)的特征。AcMNPV感染的家蚕细胞表现为细胞病理学改变，细胞增殖能力受损。免疫印迹分析表明，在AcMNPV感染的家蚕细胞中，病毒衣壳多肽的产生与常规许可的Sf21细胞相当，而囊膜融合蛋白GP64和病毒基因组DNA的产生明显低于Sf21细胞。尽管感染AcMNPV的家蚕细胞的BV产量比感染AcMNPV的Sf21细胞低一到两个数量级，但家蚕细胞支持将萌发病毒粒子(BV)产生到培养基中。在AcMNPV感染的家蚕细胞中没有检测到多角体蛋白的产生，Northern印迹分析表明，AcMNPV感染的家蚕细胞中多角体蛋白的产生缺陷是由于多角体基因表达的转录限制所致。这些结果表明，在AcMNPV感染的家蚕细胞中，病毒复制周期仅产生有限数量的后代BV进入培养基中，没有多角体基因的最佳表达。因此，本研究表明，AcMNPV感染的家蚕细胞提供了一个独特的模型系统来确定宿主依赖的病毒基因表达的细胞因素。
To characterize Autographa californica nucleopolyhedrovirus (AcMNPV) infection of the cells derived from Bombyx mori, cytopathology, cellular viability and metabolic activity, viral DNA replication, viral polypeptide synthesis, and polyhedrin gene expression were examined. AcMNPV-infected Bombyx cells displayed cytopathology and were impaird in their ability of proliferation. Immunoblot analysis showed that viral capsid polypeptides were produced in AcMNPV-infected Bombyx cells in a large quantity that was comparable to that in conventional permissive Sf21 cells, while there was a striking decrease in the production of envelope fusion protein GP64 and viral genomic DNA in Bombyx cells as compared to that in Sf21 cells. Bombyx cells supported the production of budded virions (BVs) into culture medium, although BV yield in AcMNPV-infected Bombyx cells was lower by one to two orders of magnitude than that in AcMNPV-infected Sf21 cells. No detectable amount of polyhedrin protein was produced in AcMNPV-infected Bombyx cells, and northern blot analysis demonstrated that the defect in polyhedrin production in AcMNPV-infected Bombyx cells was due to the transcriptional restriction of polyhedrin gene expression. These results indicate that in AcMNPV-infected Bombyx cells, virus replication cycle proceeds to yield a limited number of progeny BVs into culture medium, without an optimal expression of polyhedrin gene. The present study thus demonstrates that AcMNPV-infected Bombyx cells provide a unique model system to define cellular factors responsible for host-dependent viral gene expression.
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极晚因子 1 对杆状病毒多角体蛋白基因表达的控制。
影响因子：
3.7
作者：
Yang,S;Miller,LK
通讯作者：
Miller,LK
影响因子：
3.7
作者：
Oomens, AGP;Blissard, GW
通讯作者：
Blissard, GW
影响因子：
3.7
作者：
MORRIS, TD;MILLER, LK
通讯作者：
MILLER, LK
影响因子：
3.7
作者：
S. Yang;L. Miller
通讯作者：
S. Yang;L. Miller
DOI：
10.1073/pnas.93.6.2348
发表时间：
1996-03-19
影响因子：
11.1
作者：
Boyce, FM;Bucher, NLR
通讯作者：
Bucher, NLR