Antimicrobial effects of sanitizers against planktonic and sessile Listeria monocytogenes cells according to the growth phase

Antimicrobial effects of sanitizers against planktonic and sessile Listeria monocytogenes cells according to the growth phase
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DOI:
10.1016/j.femsle.2004.05.040
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发表时间:
2004-07-15
影响因子:
2.1
通讯作者:
Hébraud, M
Hébraud, M
中科院分区:
生物学4区
文献类型:
--
作者:
Chavant, P;Gaillard-Martine, B;Hébraud, M

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本研究旨在探讨单独或联合使用消毒剂对单核细胞增生李斯特菌在不同生长阶段的存活率的影响。测试的消毒剂包括:(i)乙酸(pH 5.0),(ii)NaOH(pH 12.0),(iii)10%Na2SO4,(iV)10%Na2SO4和乙酸(pH 5.0),(v)10%Na2SO4和NaOH(pH 12.0),(vi)季铵(20 ppm)和(vii)甘油单月桂酸酯(75 ppm)。结果显示,碱处理对固着细胞和非固着细胞都有很大的功效,6 h生物膜的阻力略高。季铵在杀死超过98%的细胞方面似乎非常有效,但观察到7天生物膜的抗性。其他消毒剂没有成功地完全抑制病原体,但以类似的方式对无柄细胞和无柄细胞起作用。是否更新媒介似乎并不是抵抗出现的主要原因。(C)2004年,欧洲微生物学会联合会。Elsevier B.V.出版,保留所有权利。
This study was designed to investigate the individual or combined effects of sanitizers on survival of planktonic or sessile Listeria monocytogenes cells at different phase of growth. The sanitizers tested included: (i) acetic acid (pH 5.0), (ii) NaOH (pH 12.0), (iii) 10% Na2SO4, (iV) 10% Na2SO4 and acetic acid (pH 5.0), (v) 10% Na2SO4 and NaOH (pH 12.0),.(vi) a quaternary ammonium (20 ppm) and (vii) glyceryl monolaurate (75 ppm). Results revealed a great efficacy of alkaline treatments on both sessile and planktonic cells with a slightly higher resistance of 6 h biofilms. Quaternary ammonium appeared very effective in killing more than 98% of cells, but a resistance of 7 days biofilm was observed. Other sanitizers did not succeed in inhibiting totally the pathogen but acted in a similar way on both sessile and planktonic cells. Renewing the medium or not do not seem to be the major cause of a resistance emergence. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.