Neutralizing monoclonal antibodies to the AIDS virus.
Neutralizing monoclonal antibodies to the AIDS virus.
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中和艾滋病病毒的单克隆抗体。
DOI：
10.1097/00002030-198802000-00004
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发表时间：
1988
期刊：
影响因子：
3.8
通讯作者：
R. Weiss
中科院分区：
文献类型：
--
作者：
Elaine Kinney Thomas;Jonathan N. Weber;Jan McClure;P. Clapham;Mitra C. Singhal;M. Kathleen Shriver;R. Weiss
中和性单克隆抗体（mab）的产生将允许在艾滋病病毒HIV-1上精确定位中和性表位。我们描述了LAV-1分离物包膜的7个单克隆抗体的特性。五种单克隆抗体识别gp110的中心部分，氨基酸279-472，其中四种单克隆抗体能够通过感染抑制、合胞抑制和水疱性口炎病毒（VSV）假型中和来高滴度中和HIV-1。gp41的两种单克隆抗体之一抑制合胞体的形成。gp110的中和、活细胞免疫荧光和免疫沉淀是类型特异性的，仅限于与LAV-1密切相关的HIV-1分离株。
The production of neutralizing monoclonal antibodies (MAbs) will permit the exact localization of neutralizing epitopes on the AIDS virus, HIV-1. We describe the properties of seven MAbs to the envelope of the LAV-1 isolate. Five MAbs recognise the central portion of gp110, amino acids 279-472, and four of these are capable of high-titre neutralization of HIV-1, by infection inhibition, syncytial inhibition and vesicular stomatitis virus (VSV) pseudotype neutralization. One of the two MAbs to gp41 inhibits syncytium formation. Neutralization, live cell immunofluorescence and immunoprecipitation of gp110 are type-specific and restricted to HIV-1 isolates closely related to LAV-1.