Analysis of SSRs derived from grape ESTs
Analysis of SSRs derived from grape ESTs
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DOI：
10.1007/s001220051344
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发表时间：
2000-03-01
影响因子：
5.4
通讯作者：
Henry, RJ
中科院分区：
文献类型：
--
作者：
Scott, KD;Eggler, P;Henry, RJ
从 5000 个 Vitis 表达序列标签 (EST) 的分析中分离出 124 个微卫星，存在多种二核苷酸和三核苷酸简单序列重复 (SSR) 基序。引物是为其中 16 个 SSR 设计的，并在 7 个种质上进行了测试。十六对引物中的十对产生了预期大小的 PCR 产物。所有十个功能引物在所研究的种质中都是多态性的。品种、葡萄种和相关属之间的多态性很明显，来自3'非翻译区（3'UTR）的SSR在品种水平上多态性最强，来自5'非翻译区（5'UTR）的SSR在品种和种之间多态性最强，编码序列内的SSR在种和属之间多态性最强。这些结果表明，Vitis 中源自 EST 的 SSR 非常有用，因为它们具有多态性且高度可转移。随着 EST SSR 适用于多个分类水平的研究，从 45 000 个扩展的 EST 数据库中可获得的大量 SSR（大约 1000 个）将在绘图和身份研究中具有许多潜在的应用。
One hundred and twenty four microsatellites were isolated from analysis of 5000 Vitis expressed sequence tags (ESTs), A diversity of dinucleotide and trinucleotide simple sequence repeat (SSR) motifs were present. Primers were designed for 16 of these SSRs and they were tested on seven accessions. Ten of the sixteen primer pairs resulted in PCR products of the expected size. All ten functional primers were polymorphic across the accessions studied. Polymorphisms were evident at the level of cultivars, Vitis species, and between related genera, SSRs that were from the 3' untranslated region (3'UTR) were most polymorphic at the cultivar level, the 5' untranslated region (5' UTR) SSRs were most polymorphic between cultivars and species, and those SSRs within coding sequence were most polymorphic between species and genera. These results show that EST-derived SSRs in Vitis are useful as they are polymorphic and highly transferable. With EST SSRs being applicable to studies at several taxonomic levels, the large number of SSRs (approximately 1000) that will be available from an expanded EST database of 45 000 will have many potential applications in mapping and identity research.