The nuclear proteome of chickpea (Cicer arietinum L.) reveals predicted and unexpected proteins
The nuclear proteome of chickpea (Cicer arietinum L.) reveals predicted and unexpected proteins
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DOI：
10.1021/pr060147a
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发表时间：
2006-12-01
影响因子：
4.4
通讯作者：
Chakraborty, Niranjan
中科院分区：
文献类型：
--
作者：
Pandey, Aarti;Choudhary, Mani Kant;Chakraborty, Niranjan
核蛋白是一个高度组织化的复杂网络，在细胞发育和其他生理过程中发挥着多种作用。酵母核蛋白质组约占细胞蛋白质总数的四分之一，这表明细胞核参与了许多不同的功能。为了了解植物核蛋白的复杂性，我们利用二维凝胶电泳（2-DE）建立了豆科植物鹰嘴豆的蛋白质组参考图谱。大约有600个蛋白点被检测到，LC-ESI-MS/MS分析鉴定了150个与各种细胞功能有关的蛋白质。所鉴定的蛋白质中参与信号传导和基因调控的比例最大（36%），而参与DNA复制和转录的比例为17%。鹰嘴豆核蛋白质组表明，相对于许多已知的驻留蛋白，存在一些功能未知的新核蛋白。据我们所知，这是第一个关于未测序基因组的核蛋白质组的报道。
Nuclear proteins constitute a highly organized, complex network that plays diverse roles during cellular development and other physiological processes. The yeast nuclear proteome corresponds to about one-fourth of the total cellular proteins, suggesting the involvement of the nucleus in a number of diverse functions. In an attempt to understand the complexity of plant nuclear proteins, we have developed a proteome reference map of a legume, chickpea, using two-dimensional gel electrophoresis (2-DE). Approximately, 600 protein spots were detected, and LC-ESI-MS/MS analyses led to the identification of 150 proteins that have been implicated in a variety of cellular functions. The largest percentage of the identified proteins was involved in signaling and gene regulation (36%), while 17% were involved in DNA replication and transcription. The chickpea nuclear proteome indicates the presence of few new nuclear proteins of unknown functions vis-a-vis many known resident proteins. To the best of our knowledge, this is the first report of a nuclear proteome of an unsequenced genome.