Organogenesis-stage cytochrome P450 isoforms: utilization of PCR for detection of CYP1A1 mRNA in rat conceptal tissues.
Organogenesis-stage cytochrome P450 isoforms: utilization of PCR for detection of CYP1A1 mRNA in rat conceptal tissues.
复制标题
器官发生阶段细胞色素 P450 亚型：利用 PCR 检测大鼠概念组织中的 CYP1A1 mRNA。
DOI：
10.1016/0006-291x(91)91804-l
复制
发表时间：
1991
影响因子：
3.1
通讯作者：
Juchau,MR
中科院分区：
文献类型：
--
作者：
Yang,HY;Zelus,BD;Juchau,MR
利用逆转录酶联聚合酶链反应，我们分析了三组大鼠的孕体组织表达细胞色素P4501 A1（CYP 1A 1）mRNA的能力在器官发生的畸形敏感阶段。内脏卵黄囊，外胎盘锥和胚胎适当的每一个进行了研究，在妊娠第12天，在子宫内的3-甲基胆蒽作为诱导剂的事先暴露和不。使用两组区分性寡核苷酸引物，仅在3-甲基胆蒽预暴露的孕体组织中可检测到明确的、可重复的信号。内脏volk囊组织观察到最高强度的信号，胚胎本身的组织观察到最低强度的信号。使用Southern印迹与内部寡核苷酸探针杂交来验证扩增的cDNA的特异性。结果表明，大鼠器官发生阶段的妊娠组织将表达CYP 1A 1 mRNA的环境反式调节剂的反应。
Utilizing the reverse transcriptase-linked polymerase chain reaction, we analyzed the capacity of three groups of rat conceptal tissues to express cytochrome P4501A1 (CYP1A1) mRNA during the dysmorphogenesis-sensitive stage of organogenesis. The visceral yolk sac, ectoplacental cone and embryo proper each were investigated on day 12 of gestation with and without prior exposure in utero to 3-methylcholanthrene as inducing agent. With two sets of discriminating oligonucleotide primers, definitive, reproducible signals were detectable only in tissues from 3-methylcholanthrene preexposed conceptuses. Signals of highest intensity were observed with visceral volk sac tissues and signals of lowest intensity were observed with tissues of the embryo per se. Specificities of the amplified cDNAs were verified using Southern blotting with hybridization to an internal oligonucleotide probe. The results indicate that organogenesis-stage conceptal tissues of the rat will express CYP1A1 mRNA in response to environmental transregulating agents.