Identification of seven differentially displayed transcripts in human primary and matched metastatic head and neck squamous cell carcinoma cell lines: Implications in metastasis and/or radiation response
Identification of seven differentially displayed transcripts in human primary and matched metastatic head and neck squamous cell carcinoma cell lines: Implications in metastasis and/or radiation response
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DOI：
10.1016/s0964-1955(96)00065-6
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发表时间：
1997-05-01
期刊：
影响因子：
4.8
通讯作者：
Kasid, U
中科院分区：
文献类型：
--
作者：
Patel, S;Wang, FH;Kasid, U
通过 mRNA 的差异显示和 Northern 印迹杂交，在两个原发性 (A's) 和两个匹配的转移性 (B's) 头颈鳞状细胞癌 (HNSCC) 细胞系中检查差异基因表达模式。使用的三种细胞系（PCI-04A、PCI-04B和PCP-06A）是在治疗前独立建立的，而一种细胞系（PCI-06B）是从放射治疗后的复发肿瘤建立的。共鉴定出7个差异表达的mRNA克隆，其中6个克隆是通过PCI-06A细胞与PCI-06B细胞（SCC-S1a/b、SCC-lc和SCC-SZ、PCI-06B；SCC-S3至SCC-SS、PCI-06A）比较获得的，1个克隆是从PCI-04A和PCI-04B匹配（SCC-Sa、PCI-04B）获得的。根据DNA数据库搜索与已知序列的同源性，七个部分cDNA克隆中的六个（SCC-S1a/b、SCC-lc、SCC-S2至SCC-S4和SCC-Sa）可能代表新基因，而一个cDNA克隆（SCC-SS）显示与HLA II类抗原基因（DPW2β链）显着同源。通过RNA印迹分析，与匹配的对应物相比，这七个克隆中的每一个都在原始细胞系中显示出优先表达。转录本的大小范围约为 7.0 Kb 至 0.5 Kb。有趣的是，与原发肿瘤来源的细胞系相比，SCC-Sa 克隆在两种转移细胞系中均优先表达。我们的结论是，SCC-Sa 基因可能更常见地参与肿瘤转移，而其他基因（SCC-S1a/b、SCC-Slc、SCC-S2-SCC-S5）的表达可能与 HNSCC 的转移和/或对电离辐射的反应有关。 (C) 1997 爱思唯尔科学有限公司。
The patterns of differential gene expression were examined in two primary (A's) and two matched-metastatic (B's) head and neck squamous cell carcinoma (HNSCC) cell lines by differential display of mRNAs and northern blot hybridisation. Three cell lines used (PCI-04A, PCI-04B and PCP-06A) were established independently prior to therapy, whereas one cell line (PCI-06B) was established from a recurrent tumour after radiation therapy. A total of seven differentially displayed mRNA clones were identified, of which six clones were obtained by comparison of PCI-06A cells with PCI-06B cells (SCC-S1a/b, SCC-lc, and SCC-SZ, PCI-06B; SCC-S3 to SCC-SS, PCI-06A), and one clone was obtained from the PCI-04A and PCI-04B match (SCC-Sa, PCI-04B). Based on the DNA database search for homology to the known sequences, six of the seven partial cDNA clones (SCC-S1a/b, SCC-lc, SCC-S2 to SCC-S4, and SCC-Sa) may represent novel genes, whereas one cDNA clone (SCC-SS) shows significant homology to the HLA class II antigen gene (DPW2 beta chain). Each of the seven clones revealed preferential expression by northern blotting in the cell Line of origin as compared to the matched counterpart. The transcripts ranged in size from approximately 7.0 Kb to 0.5 Kb. Interestingly, the SCC-Sa clone was preferentially expressed in both metastatic cell lines compared to the primary tumour-derived cell lines. We conclude that the SCC-Sa gene may be more commonly involved in tumour metastasis, whereas expression of the other genes (SCC-S1a/b, SCC-Slc, SCC-S2-SCC-S5) may be associated with metastasis and/or response of HNSCC to ionising radiation. (C) 1997 Elsevier Science Ltd.