Building proteomic tool boxes to monitor MHC class I and class II peptides
Building proteomic tool boxes to monitor MHC class I and class II peptides
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DOI：
10.1002/pmic.201600061
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发表时间：
2017-01-01
期刊：
影响因子：
3.4
通讯作者：
Lill, Jennie R.
中科院分区：
文献类型：
--
作者：
Schumacher, Frances-Rose;Delamarre, Lelia;Lill, Jennie R.
主要组织相容性复合体I类（MHCI）和II类（MHCII）肽强有力地调节T细胞免疫，在哺乳动物产生有效的抗肿瘤和抗病毒免疫应答中发挥重要作用。表征这些MHCI或MHCII呈现的肽可以帮助产生治疗方法，提供T细胞介导的生物标志物信息，提供对疾病进展的洞察，并减少工程生物疗法的不良抗药物副作用。在这里，我们探索了通常用于发现MHCI-和MHCI-呈递肽的工具和技术。我们描述了增强这些肽表征的互补策略，以及用于预测和表征MHCI-和MHCI-呈递表位的信息学工具。讨论了分离mhc呈现肽的方法的演变，以及可用于其表征的质谱工作流程。我们提供了这一领域的发展方向，以及这些工具如何适用于发现和监测各种情况下的表位。
Major histocompatibility complex Class I (MHCI) and Class II (MHCII) presented peptides powerfully modulate T cell immunity and play a vital role in generating effective anti-tumor and anti-viral immune responses in mammals. Characterizing these MHCI or MHCII presented peptides can help generate therapeutic treatments, afford information on T cell mediated biomarkers, provide insight into disease progression, and reduce adverse anti-drug side effects from engineered biotherapeutics. Here, we explore the tools and techniques commonly employed to discover both MHCI- and MHCII-presented peptides. We describe complementary strategies that enhance the characterization of these peptides and the informatics tools employed for both predicting and characterizing MHCI- and MHCII-presented epitopes. The evolution of methodologies for isolating MHC-presented peptides is discussed, as are the mass spectrometric workflows that can be employed for their characterization. We provide a perspective on where this field is headed, and how these tools may be applicable to the discovery and monitoring of epitopes in a variety of scenarios.