Enrichment of viable lymphocytes in defined cycle phases by sorting on the basis of pulse width of axial light extinction.
Enrichment of viable lymphocytes in defined cycle phases by sorting on the basis of pulse width of axial light extinction.
复制标题
通过根据轴向消光脉冲宽度进行分类，富集指定周期阶段的活淋巴细胞。
DOI：
10.1002/cyto.990030107
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Cambier,JC
中科院分区：
文献类型：
--
作者：
Monroe,JG;Havran,WL;Cambier,JC
在有丝分裂原刺激的小鼠淋巴细胞中，细胞大小和周期状态之间存在相关性。细胞周期分析采用多参数细胞荧光分光光度分析。细胞大小通过测量轴向消光的“飞行时间”来确定。直径为5.0-6.0μm的细胞主要分布在G0。在刺激的细胞中，直径6.0~7.0μm的细胞以早、晚G1期为主(80%)，也有少量的S期细胞。在直径7.5~12μm的淋巴细胞中，以G2+M细胞为主。该方法提供了一种分离细胞周期特定时相的淋巴细胞的方法，适用于后续的功能研究。
A correlation between cell size and cycle state is demonstrated for mitogen stimulated murine lymphocytes. Cell cycle analysis was performed using multiparameter cytofluorometric analysis of acridine orange stained cells. Cell size was determined by measuring “time of flight” of axial light extinction. Cells with diameters of 5.0–6.0 μm are shown to be predominately in G0. Among stimulated cells, those 6.0–7.0 μm in diameter are predominately early and late G1(80%) with some S phase cells. Cells in G2+ M were found to predominate among lymphocytes with diameters of 7.5–12 μm. This approach provides a method for isolation of lymphocytes in specific phases of the cell cycle which are suitable for subsequent functional studies.