Isolation of human NURF:: a regulator of Engrailed gene expression
Isolation of human NURF:: a regulator of Engrailed gene expression
复制标题
DOI：
10.1093/emboj/cdg582
复制
发表时间：
2003-11-17
期刊：
影响因子：
11.4
通讯作者：
Shiekhattar, R
中科院分区：
文献类型：
--
作者：
Barak, O;Lazzaro, MA;Shiekhattar, R
染色质结构的修饰是发育基因表达的重要调控机制。哺乳动物 ISWI 基因 SNF2H 和 SNF2L 的差异表达表明它们具有不同的发育作用。在这里，我们描述了第一个含有人类 SNF2L 的复合物的纯化和表征。亚基组成表明它代表果蝇核小体重塑因子（NURF）复合物的人类直系同源物。人类 NURF (hNURF) 在大脑中丰富，我们证明它可以调节人类 Engrailed，这是一种调节中后脑神经元发育的同源域蛋白。此外，我们发现 hNURF 可以增强细胞培养物中的神经突生长。综上所述，我们的数据表明 ISWI 复合物在神经元生长中发挥作用。
The modification of chromatin structure is an important regulatory mechanism for developmental gene expression. Differential expression of the mammalian ISWI genes, SNF2H and SNF2L, has suggested that they possess distinct developmental roles. Here we describe the purification and characterization of the first human SNF2L-containing complex. The subunit composition suggests that it represents the human ortholog of the Drosophila nucleosome-remodeling factor (NURF) complex. Human NURF (hNURF) is enriched in brain, and we demonstrate that it regulates human Engrailed, a homeodomain protein that regulates neuronal development in the mid-hindbrain. Furthermore, we show that hNURF potentiates neurite outgrowth in cell culture. Taken together, our data suggess a role for an ISWI complex in neuronal growth.