RENIN-ANGIOTENSIN SYSTEM IN PRIMITIVE BONY FISHES AND A HOLOCEPHALIAN
RENIN-ANGIOTENSIN SYSTEM IN PRIMITIVE BONY FISHES AND A HOLOCEPHALIAN
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DOI：
10.1152/ajplegacy.1973.224.4.950
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发表时间：
1973-01-01
影响因子：
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通讯作者：
SAWYER, WH
中科院分区：
文献类型：
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作者：
NISHIMURA, H;OGAWA, M;SAWYER, WH
材料和方法鱼和来源。1)Coelacanth; Latimeria chaluminosa（Grande Comore）通过英国皇家学会的Coelacanth研究委员会获得。2)两种dipnoans;非洲肺鱼Protopterus aethiopicus（乌干达）和南美肺鱼Lepidosiren paradoxa（巴西、哥伦比亚或秘鲁）是商业购买的。3)全骨类2种;长吻雀鳝Lepisosteus osseus和弓鳍Amia calva（五大湖）由韦恩州立大学生物系和纽约的奥斯本海洋科学实验室提供。4)与M.的奥斯本实验室合作获得了三种软骨鱼：短吻鲟鱼（五大湖）。海洋科学，纽约。尼罗河鱼PoZypterus seneparus和芦苇鱼Calamoichthys calabaricus（乌干达）购自Tiburon Co. Biomarine Preparations，西雅图。5)在华盛顿大学星期五港实验室的合作下，当地渔民收集了一种全头动物，即Hydrolagus colliei（圣胡安群岛，华盛顿）。除了腔棘鱼和鼠鱼，所有的鱼都来自FW。FW鱼在使用前已在水族箱中保存数月。腔棘鱼和鼠鱼在捕获当天被杀死。收集血液和肾脏。通过浸入0.01-0.02%三-凯恩甲磺酸盐（Finquel，Ayerst Laboratories）中麻醉除腔棘鱼之外的所有鱼。通过插入主动脉如腹主动脉、肺动脉或肺动脉的插管抽取血液。
MATERIALS AND METHODSFishes and sources. 1) Coelacanth; Latimeria chalumnae (Grande Comore) was obtained through the Coelacanth Research Committee of the British Royal Society. 2) Two species of dipnoans; the African lungfish Protopterus aethiopicus (Uganda) and the South American lungfish Lepidosiren paradoxa (Brazil, Colombia, or Peru) were purchased commercially. 3) Two species of holosteans; the longnose gar Lepisosteus osseus and the bowfin Amia calva (Great Lakes) were kindly supplied by the Department of Biology, Wayne State University, and by the Osborn Laboratories of Marine Sciences, New York. 4) Three species of chondrosteans; the shortnose sturgeon Acipenser brevirostris (Great Lakes) was obtained with cooperation of the Osborn Laboratories of M. arine Sciences, New York. The Nile bichir PoZypterus senegalus and the reed fish Calamoichthys calabaricus (Uganda) were purchased through Tiburon Co. Biomarine Preparations, Seattle. 5) A species of holocephalian, the ratfish Hydrolagus colliei (San Juan Archipelago, Washington) was collected by local fishermen with the cooperation of the Friday Harbor Laboratories, University of Washington. Fishes are all from FW except the coelacanth and the ratfish. The FW fishes had been kept in aquaria for several months before use. Coelacanth and ratfish were killed on the day of capture. Collection of blood and kidney. All fishes except the coelacanth were anesthetized by-immersion in 0.01-0.02% tri-Caine methane sulfonate (Finquel, Ayerst Laboratories). Blood was drawn through a cannula inserted into a major artery such as the ventral aorta, pulmonary artery, or