PacMYBA, a sweet cherry R2R3-MYB transcription factor, is a positive regulator of salt stress tolerance and pathogen resistance
PacMYBA, a sweet cherry R2R3-MYB transcription factor, is a positive regulator of salt stress tolerance and pathogen resistance
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PacMYBA 是一种甜樱桃 R2R3-MYB 转录因子，是盐胁迫耐受性和病原体抗性的正调节因子
DOI：
10.1016/j.plaphy.2017.01.015
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发表时间：
2017-03-01
影响因子：
6.5
通讯作者：
Li, Tianhong
中科院分区：
文献类型：
--
作者：
Shen, Xinjie;Guo, Xinwei;Li, Tianhong
植物R2 R3-MYB转录因子在植物逆境响应中起着重要作用。我们以前从甜樱桃cv. Hong Deng，命名为PacMYBA（GenBank登录号KF 974774）。为了探索PacMYBA在植物逆境应答中的作用，我们在转基因拟南芥中异源表达PacMYBA。在以前的研究中，我们证明PacMYBA主要定位于细胞核，并可以由脱落酸（阿坝）诱导。对PacMYBA启动子序列的分析表明，它含有几个与胁迫相关的顺式元件。QPCR结果表明，甜樱桃叶片中的PacMYBA受盐、水杨酸（SA）和茉莉酸（JA）的诱导。异源表达PacMYBA的转基因拟南芥植物表现出耐盐性增强和对假单胞菌注射致病菌的抗性增强。番茄（Pst）DC 3000侵染。过量表达PacMYBA降低了转基因拟南芥的渗透势（OP），增加了游离脯氨酸含量，并增加了过氧化物酶的含量。此外，PacMYBA的过表达也影响了转基因植株中盐胁迫和病原菌防御相关基因的表达水平。这些结果表明，PacMYBA是盐胁迫耐受性和病原菌抗性的正向调节剂。(C)2017 Elsevier Masson SAS。All rights reserved.
Plant R2R3-MYB transcription factors play crucial roles in stress responses. We previously isolated a R2R3-MYB homolog from sweet cherry cv. Hong Deng, designated PacMYBA (GenBank accession No. KF974774). To explore the role of PacMYBA in the plant stress response, we heterologously expressed PacMYBA in transgenic Arabidopsis thaliana plants. In a previous study, we demonstrated that PacMYBA is mainly localized to the nucleus and could be induced by abscisic acid (ABA). Analysis of the promoter sequence of PacMYBA revealed that it contains several stress-related cis-elements. QPCR results showed that PacMYBA is induced by salt, salicylic (SA), and jasmonic acid JA) in sweet cherry leaves. Transgenic Arabidopsis plants heterologously expressing PacMYBA exhibited enhanced salt-tolerance and increased resistance to Pseudomonas syringe pv. tomato (Pst) DC3000 infection. Overexpression of PacMYBA decreased the osmotic potential (OP), increased the free proline content, and increased the peroxidase content in transgenic Arabidopsis plants. Furthermore, overexpression of PacMYBA also affected the expression levels of salt stress-and pathogen defense-related genes in the transgenic plants. These results indicate that PacMYBA is a positive regulator of salt stress tolerance and pathogen resistance. (C) 2017 Elsevier Masson SAS. All rights reserved.