Next-generation sequencing technologies and their impact on microbial genomics
Next-generation sequencing technologies and their impact on microbial genomics
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DOI：
10.1093/bfgp/els062
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发表时间：
2013-09-01
影响因子：
4
通讯作者：
O'Toole, Paul W.
中科院分区：
文献类型：
--
作者：
Forde, Brian M.;O'Toole, Paul W.
下一代测序技术通过提供传统毛细管测序仪的低成本、高通量替代品，在基因组研究领域产生了巨大影响。这些新的测序方法已经超越了它们最初的范围，现在提供了一系列基于实用性的应用，这使得对微生物基因组的结构和内容进行比以前更全面的分析成为可能。随着第三代测序技术的商业化迫在眉睫，我们讨论了当前下一代测序方法的应用，并探讨了它们对微生物基因组研究的影响和贡献。
Next-generation sequencing technologies have had a dramatic impact in the field of genomic research through the provision of a low cost, high-throughput alternative to traditional capillary sequencers. These new sequencing methods have surpassed their original scope and now provide a range of utility-based applications, which allow for a more comprehensive analysis of the structure and content of microbial genomes than was previously possible. With the commercialization of a third generation of sequencing technologies imminent, we discuss the applications of current next-generation sequencing methods and explore their impact on and contribution to microbial genome research.