Relatedness of medically important strains of Saccharomyces cerevisiae as revealed by phylogenetics and metabolomics
Relatedness of medically important strains of Saccharomyces cerevisiae as revealed by phylogenetics and metabolomics
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DOI：
10.1002/yea.1601
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发表时间：
2008-07-01
期刊：
影响因子：
2.6
通讯作者：
Roberts, Ian N.
中科院分区：
文献类型：
--
作者：
MacKenzie, Donald A.;Defernez, Marianne;Roberts, Ian N.
10个医学上重要的酵母菌株，包括6个临床分离的酿酒酵母和4个益生菌菌株的布拉酵母，其特征在于在遗传和代谢水平，并与非医疗，商业酵母菌株用于烘焙和酿酒。菌株进行了比较，遗传指纹，扩增片段长度多态性（AFLP）分析，核糖体DNA ITS 1测序和代谢足迹，直接注射质谱（DIMS）和气相色谱-飞行时间质谱（GC-ToF-MS）。总体而言，与非医疗菌株相比，临床分离株分为不同的分组，菌株之间在遗传和代谢水平上具有良好但不完美的相关性。益生菌菌株S.在治疗上用于治疗人胃肠道疾病的布拉氏酵母在遗传和代谢上都显示出紧密的聚集。代谢组学被认为是有价值的分类工具，并作为一种手段，调查基因型和表型之间的异常联系。当比较临床、益生菌和非医疗菌株的三个主要组时，鉴定了关键的区别性代谢物，包括海藻糖、肌醇、乳酸、富马酸和甘油3-磷酸等分子。这项研究证实了一部分临床分离株与烘焙或益生菌菌株之间的联系，但也强调了临床菌株在基因组和代谢水平上的多样性。版权所有（c）2008约翰威利父子有限公司。
Ten medically important Saccharomyces strains, comprising six clinical isolates of Saccharomyces cerevisiae and four probiotic strains of Saccharomyces boulardii, were characterized at the genetic and metabolic level and compared with non-medical, commercial yeast strains used in baking and wine-making. Strains were compared by genetic fingerprinting using amplified fragment length polymorphism (AFLP) analysis, by ribosomal DNA ITS1 sequencing and by metabolic footprinting using both direct injection mass spectrometry (DIMS) and gas chromatography-time of flight-mass spectrometry (GC-ToF-MS). Overall, the clinical isolates fell into different groupings when compared with the non-medical strains, with good but not perfect correlation amongst strains at both the genetic and metabolic levels. Probiotic strains of S. boulardii that are used therapeutically to treat human gastrointestinal tract disorders showed tight clustering both genetically and metabolically. Metabolomics was found to be of value both as a taxonomic tool and as a means to investigate anomalous links between genotype and phenotype. Key discriminatory metabolites were identified when comparing the three main groups of clinical, probiotic and non-medical strains and included molecules such as trehalose, myoinositol, lactic acid, fumaric acid and glycerol 3-phosphate. This study confirmed the link between a subset of clinical isolates and baking or probiotic strains but also highlighted that in general the clinical strains were more diverse at both the genomic and metabolic levels. Copyright (c) 2008 John Wiley & Sons, Ltd.