A 1.8 kb GFAP-promoter fragment is active in specific regions of the embryonic CNS
A 1.8 kb GFAP-promoter fragment is active in specific regions of the embryonic CNS
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DOI：
10.1016/s0925-4773(01)00460-9
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发表时间：
2001-09-01
影响因子：
2.6
通讯作者：
Nistér, M
中科院分区：
文献类型：
--
作者：
Andræ, J;Bongcam-Rudloff, E;Nistér, M
胶质纤维酸性蛋白(GFAP)是星形胶质细胞的主要细胞骨架蛋白(J.8(1985)203)，并且传统上被认为是星形胶质细胞的特异性标记，为了识别早期的胶质前体细胞，我们利用1.8kb的人GFAP 5‘片段创建了GFAP启动子-LacZ转基因小鼠。在胚胎9.5天的神经上皮细胞中首次检测到转基因的表达。此外，在发育中的端脑的脑室带、小脑原基、三叉神经节和放射状胶质细胞中也有发现。随后，在脑桥、脑干和胶质细胞限制区可见散在的β-半乳糖(+)细胞。结果表明，GFAP活性在中枢神经系统(CNS)发育过程中是以区域特异性的方式调节的，并且该基因在不同类型的细胞中是独立启动的。(C)2001爱思唯尔爱尔兰科学有限公司。保留所有权利。
The intermediate filament glial fibrillary acidic protein (GFAP) constitutes the major cytoskeletal protein in astrocytes (J. Neuroimmunol. 8 (1985) 203) and is traditionally referred to as a specific marker for astrocytes, To identify early glial precursors, we created GFAPpromoter-lacZ transgenic mice, using a 1.8 kb 5 ' fragment of human GFAP. The expression of the transgene was first detected in the neuroepithelium at embryonic day 9.5. It was further found in the ventricular zone of the developing telencephalon, in the cerebellar primordium, trigeminal ganglia, and radial glia. Later, scattered beta -gal(+) cells were seen in pons, brain stem and glia limitans. The results indicate that GFAP activity is regulated in a region-specific manner during central nervous system (CNS) development and that the gene is turned on in different cell types independently. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.