Isolation and fine mapping of Rps6: an intermediate host resistance gene in barley to wheat stripe rust.
Isolation and fine mapping of Rps6: an intermediate host resistance gene in barley to wheat stripe rust.
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DOI：
10.1007/s00122-015-2659-x
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发表时间：
2016-04
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影响因子：
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通讯作者：
Moscou MJ
中科院分区：
文献类型：
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作者：
Dawson AM;Ferguson JN;Gardiner M;Green P;Hubbard A;Moscou MJ
我们分离了大麦对条锈菌的寄主和非寄主抗性。霍尔德兰小麦属。我们对大麦7H染色体Rps6进行了分离、精细定位和物理定位。如果一种植物的所有已知基因类型对该病原菌的所有已知菌株都具有抗性，则该植物可被认为是一种病原菌的非寄主。然而，如果少数基因类型对病原菌的某些已知分离物敏感，这种植物可以被认为是中间寄主。大麦是条锈菌的中间寄主。小麦条锈菌(Tritici，PST)是小麦条锈病的病原。我们想了解PST抗性的遗传结构，并确定是否存在与寄主条锈菌f.sp.的抗性重叠。荷尔德氏(PSH)。我们将PST抗性定位在7H染色体上，表明寄主和中间寄主的抗性在遗传上是分离的。因此，我们将该抗病基因座命名为Rps6。我们对F2：3家系进行了表型选择和基因选择，分离到Rps6，并将该基因座精细定位到0.1 cM的区域。将Rps6基因座锚定在大麦物理图谱上，将该区域置于一个单一的指纹重叠群上，该重叠群横跨267 kb的物理区域。现在正在努力对最小平铺路径进行排序，并划定容纳RPS6的物理区域。这将促进更多的标记开发，并允许识别该区域的候选基因。本文的在线版本(doi：10.1007/s00122-0152659-x)包含补充材料，授权用户可以使用。
We uncouple host and nonhost resistance in barley toPuccinia striiformisff. spp.hordeiandtritici. We isolate, fine map, and physically anchorRps6to chromosome 7H in barley. A plant may be considered a nonhost of a pathogen if all known genotypes of a plant species are resistant to all known isolates of a pathogen species. However, if a small number of genotypes are susceptible to some known isolates of a pathogen species this plant may be considered an intermediate host. Barley (Hordeum vulgare) is an intermediate host for Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust. We wanted to understand the genetic architecture underlying resistance to Pst and to determine whether any overlap exists with resistance to the host pathogen, Puccinia striiformis f. sp. hordei (Psh). We mapped Pst resistance to chromosome 7H and show that host and intermediate host resistance is genetically uncoupled. Therefore, we designate this resistance locus Rps6. We used phenotypic and genotypic selection on F2:3 families to isolate Rps6 and fine mapped the locus to a 0.1 cM region. Anchoring of the Rps6 locus to the barley physical map placed the region on a single fingerprinted contig spanning a physical region of 267 kb. Efforts are now underway to sequence the minimal tiling path and to delimit the physical region harboring Rps6. This will facilitate additional marker development and permit identification of candidate genes in the region. The online version of this article (doi:10.1007/s00122-015-2659-x) contains supplementary material, which is available to authorized users.