ISOLATION, CLONING, AND SEQUENCING OF SIMIAN FOAMY VIRUSES FROM CHIMPANZEES (SFVCPZ) - HIGH HOMOLOGY TO HUMAN FOAMY VIRUS (HRV)
ISOLATION, CLONING, AND SEQUENCING OF SIMIAN FOAMY VIRUSES FROM CHIMPANZEES (SFVCPZ) - HIGH HOMOLOGY TO HUMAN FOAMY VIRUS (HRV)
复制标题
DOI：
10.1006/viro.1994.1285
复制
发表时间：
1994-06-01
期刊：
影响因子：
3.7
通讯作者：
LUCIW, PA
中科院分区：
文献类型：
--
作者：
HERCHENRODER, O;RENNE, R;LUCIW, PA
从黑猩猩 B 细胞系中回收了几种独立的猿猴泡沫病毒 (SFV) 分离株。对一种分离株进行了分子克隆和测序，命名为 SFVcpz。此外，还部分克隆了独立的黑猩猩泡沫病毒分离株SR 6型（SFV-6）的基因组。 SFVcpz 原病毒的长度为 13,246 个碱基对 (bp)。它的两侧是长末端重复序列 (LTR)，编码基因 gag、pol、env、转录反式激活子 taf 和第二个 3' 开放阅读框 (orf4)，源自 SFV-6 pol、env 和 orf-2 基因的分子克隆的 DNA 序列与 SFVcpz 的 DNA 序列几乎相同。 SFVcpz 的 DNA 和推导的蛋白质序列与人类泡沫病毒 (H FV) 显示出高度同源性，而来自恒河猴的 SFV-1 和来自非洲绿猴的 SFV-3 是系统发育上距离较远的病毒。 SFVcpz和HFV的相应基因之间的氨基酸同源性范围在taf基因的86%和pol基因的95%之间。 SFVcpz 的 taf 和 pol 与 SFV-1 和 SFV-8 的比较分别显示 40% 和 78% 的同源性。 SFVcpz LTR 由 1760 bp 组成，与 SFV-1 和 -3 的 LTR 大小范围相同，但明显大于已知的 HFV LTR。这些比较表明，HFV LTR 中缺失了大约 500 bp 长的区域。我们还分离并测序了野生型 HFV 原病毒的 LTR，其与 SFVcpz LTR 具有高度同源性，没有重大缺口。根据本报告中的序列比较，灵长类泡沫病毒可能被排列成不同的簇，其中 SFVcpz 和 HFV 形成一个簇，SFV-1 和 SFV-3 作为两个独特簇的原型，(C) 1994 年学术出版社。公司
Several independent isolates of simian foamy viruses (SFV) were recovered from chimpanzee B-cell lines. One isolate, designated SFVcpz, was molecularly cloned and sequenced. In addition, the genome of SR type 6 (SFV-6), an independent chimpanzee foamy virus isolate, was partially cloned. The SFVcpz provirus is 13,246 base pairs (bp) long. It is flanked by long terminal repeats (LTRs) and encodes the genes gag, pol, env, the transcriptional transactivator taf, and a second 3' open reading frame (orf4), DNA sequences of molecular clones derived from the pol, env, and orf-2 genes of SFV-6 are almost identical to those of SFVcpz. DNA and deduced protein sequences of SFVcpz show high homologies to human foamy virus (H FV), whereas both SFV-1 from a rhesus macaque and SFV-3 from an Af rican green monkey are phylogenetically further distant viruses. Amino acid homologies between corresponding genes of SFVcpz and HFV range between 86% for the taf gene and 95% for the pol gene. Comparisons of taf and pol of SFVcpz with SFV-1 and SFV-8 Show 40 and 78% homology, respectively. The SFVcpz LTR consists of 1760 bp and is in the same size range as the LTRs of SFV-1 and -3, but significantly larger than the known HFV LTR. These comparisons reveal that a region approximately 500 bp long is missing in the HFV LTR. We also isolated and sequenced an LTR of a wild-type HFV provirus which aligns with high homology to the SFVcpz LTR without major gaps. Based on sequence comparisons in this report, primate foamy viruses may be arranged into different clusters with SFVcpz and HFV forming one cluster and SFV-1 and SFV-3 as prototypes for two unique clusters, (C) 1994 Academic Press. Inc.