Joint development in Xenopus laevis and induction of segmentations in regenerating froglet limb (spike)
Joint development in Xenopus laevis and induction of segmentations in regenerating froglet limb (spike)
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DOI：
10.1002/dvdy.20484
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发表时间：
2005-08-01
影响因子：
2.5
通讯作者：
Ide, H
中科院分区：
文献类型：
--
作者：
Satoh, A;Suzuki, M;Ide, H
在非洲爪蟾中，成年肢的截肢导致形成一个简单的（亚形态）的没有关节的穗状结构，尽管蝌蚪肢芽再生完整的肢模式。在肢体发育和再生过程中检测了一些关节标记基因的表达。Bmp中心点4和gdf中心点5在关节区表达，sox-9表达减少。虽然发育中的软骨组织良好，有bmp-4表达的软骨周细胞，但棘突软骨没有这样的结构，只显示稀疏的bmp-4表达。将BMP 4浸泡的珠应用于穗导致诱导关节样结构。这些结果表明，缺乏关节的尖峰是由于缺乏积累的细胞表达bmp-4。我们在这里首次报道的非洲爪蟾成年肢体再生的改善将使我们对表型再生有重要的认识。(c)2005 Wiley-Liss，Inc.
In Xenopus laevis, amputation of the adult limb results in the formation of a simple (hypomorphic) spike-like structure without joints, although tadpole limb bud regenerates complete limb pattern. The expression of some joint marker genes was examined in limb development and regeneration. Bmp center dot 4 and gdf center dot 5 were expressed and sox-9 expression was decreased in the joint region. Although developing cartilages were well-organized and had bmp-4 expressing perichondrocytes, the spike cartilage did not have such a structure, but only showed sparse bmp-4 expression. Application of BMP4-soaked beads to the spike led to the induction of a joint-like structure. These results suggest that the lack of joints in the spike is due to the deficiency of the accumulation of the cells that express bmp-4. Improvement of regeneration in the Xenopus adult limb that we report here for the first time will give us important insights into epimorphic regeneration. (c) 2005 Wiley-Liss, Inc.