Biomolecular screening with encoded porous-silicon photonic crystals
Biomolecular screening with encoded porous-silicon photonic crystals
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DOI：
10.1038/nmat702
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发表时间：
2002-09-01
期刊：
影响因子：
41.2
通讯作者：
Sailor, MJ
中科院分区：
文献类型：
--
作者：
Cunin, F;Schmedake, TA;Sailor, MJ
编码或标记用于高通量筛选和生物测定应用的小颗粒或小珠的策略侧重于空间分化，芯片上阵列或编码珠的随机分布。对随机阵列或流体悬浮液中的大量聚合物、金属或玻璃微珠进行编码的尝试使用了各种实体来提供编码元素（比特）——荧光分子、具有特定振动特征的分子、量子点或离散金属层。本文报道了一种光学编码微米级多孔硅纳米结构粒子的方法。我们在晶体硅中使用周期性电化学蚀刻生成多层多孔膜。这导致光子晶体具有良好的分辨和窄的光学反射率特征，其波长由蚀刻参数决定。数百万种可能的代码可以用这种方式编写。然后通过超声波破裂、机械研磨或光刻方法生产微米大小的颗粒。一种简单的基于抗体的生物测定，使用荧光标记的蛋白质，在生物学相关的介质中证明了编码策略。
Strategies to encode or label small particles or beads for use in high-throughput screening and bioassay applications focus on either spatially differentiated, on-chip arrays,,or random distributions of encoded beads,. Attempts to encode large numbers of polymeric, metallic or glass beads in random arrays or in fluid suspension have used a variety of entities to provide coded elements (bits)—fluorescent molecules, molecules with specific vibrational signatures,, quantum dots, or discrete metallic layers. Here we report a method for optically encoding micrometre-sized nanostructured particles of porous silicon. We generate multilayered porous films in crystalline silicon using a periodic electrochemical etch. This results in photonic crystals with well-resolved and narrow optical reflectivity features, whose wavelengths are determined by the etching parameters. Millions of possible codes can be prepared this way. Micrometre-sized particles are then produced by ultrasonic fracture, mechanical grinding or by lithographic means. A simple antibody-based bioassay using fluorescently tagged proteins demonstrates the encoding strategy in biologically relevant media.