Unravelling mitochondrial retrograde regulation in the abiotic stress induction of rice ALTERNATIVE OXIDASE 1 genes
Unravelling mitochondrial retrograde regulation in the abiotic stress induction of rice ALTERNATIVE OXIDASE 1 genes
复制标题
揭示水稻替代氧化酶 1 基因非生物胁迫诱导中的线粒体逆行调节
DOI：
10.1111/pce.12013
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发表时间：
2013-04-01
影响因子：
7.3
通讯作者：
Yang, Jian-Bo
中科院分区：
文献类型：
--
作者：
Li, Chun-Rong;Liang, Dan-Dan;Yang, Jian-Bo
线粒体逆行调节(MRR)是线粒体信号的转导，介导核基因的表达。目前还不清楚MRR是否是植物非生物胁迫反应的共同调控机制。在本研究中，我们分析了水稻OsAOX1基因的早期非生物胁迫响应，并选择OsAOX1a和OsAOX1b的诱导(OsAOX1a/b)作为胁迫诱导MRR研究的工作模型。我们发现产生超氧阴离子(O2中心点)的化学物质甲基紫精的诱导作用强于过氧化氢(H_2O_2)。ROS清除剂的加入表明，通过消除O2中心点-而降低了胁迫诱导。此外，胁迫的诱导不依赖于叶绿体或细胞质来源的O2中心点。接下来，我们产生了在不同亚细胞位置过度表达超氧化物歧化酶(SOD)基因的转基因植物。结果表明，只有线粒体超氧化物歧化酶(OsMSD)特异性地减弱了OsAOX1a/b的应激诱导。因此，我们的研究结果表明，非生物胁迫启动了OsAOX1a/b上的MRR，线粒体O2中心点参与了这一过程。
Mitochondrial retrograde regulation (MRR) is the transduction of mitochondrial signals to mediate nuclear gene expression. It is not clear whether MRR is a common regulation mechanism in plant abiotic stress response. In this study, we analysed the early abiotic stress response of the rice OsAOX1 genes, and the induction of OsAOX1a and OsAOX1b (OsAOX1a/b) was selected as a working model for the stress-induced MRR studies. We found that the induction mediated by the superoxide ion (O2 center dot-)-generating chemical methyl viologen was stronger than that of hydrogen peroxide (H2O2). The addition of reactive oxygen species (ROS) scavengers demonstrated that the stress induction was reduced by eliminating O2 center dot-. Furthermore, the stress induction did not rely on chloroplast- or cytosol-derived O2 center dot-. Next, we generated transgenic plants overexpressing the superoxide dismutase (SOD) gene at different subcellular locations. The results suggest that only the mitochondrial SOD, OsMSD, attenuated the stress induction of OsAOX1a/b specifically. Therefore, our findings demonstrate that abiotic stress initiates the MRR on OsAOX1a/b and that mitochondrial O2 center dot is involved in the process.