The cultured human cervical epithelium a new model for studying paracellular transport
The cultured human cervical epithelium a new model for studying paracellular transport
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DOI：
10.1016/s1071-5576(96)00028-7
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发表时间：
1996-09-01
期刊：
影响因子：
--
通讯作者：
Gorodeski, GI
中科院分区：
文献类型：
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作者：
Gorodeski, GI
目的：人宫颈细胞在具有较高细胞旁通透性的滤器上形成融合培养物。我们试图确定响应于细胞旁阻力调节剂的渗透性的急性和可逆变化在多大程度上影响侧向细胞间隙（R（LIS））或紧密连接（R（TJ））的阻力。通过测量物理/化学条件的组合的影响来实验性地处理该问题，所述物理/化学条件对细胞旁阻力的作用是相对较好理解的，流体静压梯度、中等张力和中等钙浓度的变化）。相对于跨上皮电阻（TER）和上皮细胞对三磺酸吡喃（Ppyr）的渗透性的变化，测定细胞旁渗透性的变化。R（TJ）的变化相对于跨上皮阳离子选择性的变化来确定，如从稀释potential.RESULTS的变化所确定的：1）低细胞外钙消除TER并降低跨上皮阳离子选择性（即，降低R（TJ））。2)由流体静压梯度和介质张力变化引起的TER变化调节TER，但对阳离子选择性没有影响（即对R（LIS）的影响）。3)低钙相关的TER降低是流体静力学和高渗梯度和低渗再现的叠加。4)腔下腔流体静力学和高渗梯度降低TER，但效果是nonadditional.CONCLUSIONS：这些结果表明，R（LIS）和R（TJ）可以选择性调制。基于对数据的定量分析，预测R（LIS）和R（TJ）构成培养的宫颈上皮的TER，并且分别贡献约25%和75%的总阻力。
OBJECTIVE: Human cervical cells form confluent cultures on filters with a relatively high degree of paracellular permeability. We sought to determine the extent to which acute and reversible changes in permeability in response to modulators of paracellular resistance affect the resistance of the lateral intercellular space (R(LIS)), or of the tight junctions (R(TJ)).METHODS: The question was approached experimentally by measuring the effects of combinations of physical/chemical conditions whose actions on the paracellular resistance are relatively well understood leg, changes in hydrostatic gradients, medium tonicity, and medium calcium concentration). Changes in paracellular permeability were determined relative to changes in transepithelial electrical resistance (TER) and to the epithelial permeability to the trisulfonic acid pyranine (Ppyr). Changes in R(TJ) were determined relative to changes in the transepithelial cation selectivity, as determined from changes in dilution potential.RESULTS: 1) Low extracellular calcium abrogates the TER and decreases transepithelial cation selectivity (ie, decreases R(TJ)). 2) Changes in TER induced by changes in hydrostatic gradients and medium tonicity modulate TER but have no effect on cation selectivity (ie, the effect war on R(LIS)). 3) Low-calcium-related decreases in TER are additive to those of hydrostatic and hypertonic gradients and hypotonic renditions. 4) Subluminal to luminal hydrostatic and hypertonic gradients decrease TER, but the effects are nonadditive.CONCLUSIONS: These results indicate that R(LIS) and R(TJ) can be selectively modulated. Based on quantitative analysis of the data it is predicated that R(LIS) and R(TJ) constitute the TER of the cultured cervical epithelium, and contribute about 25 and 75%, respectively, to the total resistance.