Genome-wide identification and expression analysis of the OSCA gene family in Pyrus bretschneideri
Genome-wide identification and expression analysis of the OSCA gene family in Pyrus bretschneideri
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DOI：
10.1139/cjps-2017-0115
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发表时间：
2018-08-01
影响因子：
1.2
通讯作者：
Wu, Juyou
中科院分区：
文献类型：
--
作者：
Gu, Xiaoyu;Wang, Peng;Wu, Juyou
植物对渗透压变化的反应对植物的生存、发育和繁殖至关重要。高渗透压诱导的胞质游离钙浓度[（Ca 2+）（i）]增加（OSCA）蛋白已被描述为植物和动物中的钙离子传感器。为了研究梨OSCA基因（PbrOSCAs）的功能，进行PbrOSCA的生物信息学和表达分析。在梨基因组中鉴定出16个PbrOSCA成员。通过序列比对和系统发育分析将PbrOSCA家族成员分为4个分支。蛋白质结构分析表明，16个PbrOSCA成员与拟南芥和水稻中的同源物具有相似的结构。PbrOSCA的多跨膜模式和离子转运孔位点是保守的，并且PbrOSCA的表达谱在组织之间和在渗透胁迫条件下变化。特别是，6个PbrOSCA的表达水平随着渗透胁迫时间的推移逐渐增加，表明PbrOSCA可能在植物渗透胁迫响应中发挥调节作用。
Responses to osmotic change are critical for plant survival, development, and reproduction. Hyperosmolality-induced cytosolic free calcium concentration [(Ca2+)(i)] increase (OSCAs) proteins have been described as osmosensors in plants and animals. To investigate functional roles of OSCA genes (PbrOSCAs) in pear (Pyrus bretschneideri Rehd.), bioinformatics, and expression analyses of the PbrOSCAs were performed. Sixteen PbrOSCA members were identified in the pear genome. PbrOSCA family members were classified into four clades by sequence alignment and phylogenetic analysis. Moreover, protein structure analysis indicated that the 16 PbrOSCA members shared similar structures with their homologues in Arabidopsis and rice. Multi-transmembrane patterns and ion transport pore sites of PbrOSCAs were conserved, and expression profiles of PbrOSCA varied among tissues and with osmotic stress conditions. In particular, expression levels of six PbrOSCAs gradually increased with time during osmotic stress, suggesting that PbrOSCAs may play regulatory roles in plant osmotic stress responses.