The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC
The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC
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DOI：
10.1016/s0092-8674(00)80261-5
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发表时间：
1997-05-30
期刊：
影响因子：
64.5
通讯作者：
Rosbash, M
中科院分区：
文献类型：
--
作者：
Berglund, JA;Chua, K;Rosbash, M
酵母剪接因子BBP（分支点桥接蛋白）在承诺复合体中高度保守的分支点序列UACUAAC附近直接与pre-mRNA相互作用。我们还发现重组蛋白能够识别UACUAAC序列。因此，BBP也是支点结合蛋白的缩写。哺乳动物剪接因子SF1是BBP同源物（mBBP）和E复合物组分，也具有分支点序列特异性。该区域在酵母和哺乳动物中的相对保守性与BBP和mBBP之间的rna结合差异密切相关，这表明BBP在这两种系统中都有助于分支点序列的定义。
The yeast splicing factor BBP (branchpoint bridging protein) interacts directly with pre-mRNA at or very near the highly conserved branchpoint sequence UACUAAC within the commitment complex. We also show that the recombinant protein recognizes the UACUAAC sequence. Therefore, BBP is also an acronym for branchpoint binding protein. The mammalian splicing factor SF1 is a BBP ortholog (mBBP) and an E complex component, and also has branchpoint sequence specificity. The relative conservation of this region in yeast and mammals correlates well with the RNA-binding differences between BBP and mBBP, suggesting that BBP contributes to branchpoint sequence definition in both systems.