Solution structure of the N-terminal A domain of the human voltage-gated Ca2+channel beta4a subunit.
Solution structure of the N-terminal A domain of the human voltage-gated Ca2+channel beta4a subunit.
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人类电压门控 Ca2 通道 beta4a 亚基 N 端 A 结构域的溶液结构。
DOI：
10.1110/ps.051894506
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Horne,WilliamA
中科院分区：
文献类型：
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作者：
Vendel,AndrewC;Rithner,ChristopherD;Lyons,BarbaraA;Horne,WilliamA
Ca2+ 通道 β 亚基调节电压依赖性 Ca2+ 通道 α1 亚基的运输和门控（打开和关闭）。根据一级序列比较，它们被认为是由五个结构域（A-E）组成的模块化结构，与膜相关鸟苷酸激酶（MAGUK）蛋白大家族相关。最近报道了β亚基核心B-D结构域的晶体结构；然而，人们对 A 和 E 结构域的结构知之甚少。 N 末端 A 结构域是一个高变区，在 Ca2+ 通道 β 亚基的四种亚型 (β1-β4) 中存在差异。此外，该结构域经过选择性剪接，在给定基因类别内创建多个 N 末端结构，这些结构对门控具有不同的影响。我们已经解决了人类 β4a 亚基 A 结构域的解结构，这是一种剪接变体，我们之前已经证明它对 Ca2+ 通道运输和门控具有 α1 亚基亚型特异性影响。
Ca2+channel β subunits regulate trafficking and gating (opening and closing) of voltage‐dependent Ca2+channel α1subunits. Based on primary sequence comparisons, they are thought to be modular structures composed of five domains (A–E) that are related to the large family of membrane associated guanylate‐kinase (MAGUK) proteins. The crystal structures of the β subunit core, B–D, domains have recently been reported; however, very little is known about the structures of the A and E domains. The N‐terminal A domain is a hypervariable region that differs among the four subtypes of Ca2+channel β subunits (β1–β4). Furthermore, this domain undergoes alternative splicing to create multiple N‐terminal structures within a given gene class that have distinct effects on gating. We have solved the solution structure of the A domain of the human β4asubunit, a splice variant that we have shown previously to have α1subunit subtype‐specific effects on Ca2+channel trafficking and gating.