Transcriptional comparison of the photogenic and non-photogenic tissues of Phrixothrix hirtus (Coleoptera: Phengodidae) and non-luminescent Chauliognathus flavipes (Coleoptera: Cantharidae) give insights on the origin of lanterns in railroad worms
Transcriptional comparison of the photogenic and non-photogenic tissues of Phrixothrix hirtus (Coleoptera: Phengodidae) and non-luminescent Chauliognathus flavipes (Coleoptera: Cantharidae) give insights on the origin of lanterns in railroad worms
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DOI：
10.1016/j.genrep.2017.02.004
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发表时间：
2017-06-01
期刊：
影响因子：
1.3
通讯作者：
Viviani, Vadim R.
中科院分区：
文献类型：
--
作者：
Amaral, Danilo T.;Silva, Jaqueline R.;Viviani, Vadim R.
在甲虫中发现生物发光是在Elateroidae超家族中发现的，包括Elateridae、Lampyridae、Rhagophthalmidae和Phengodidae家族。尽管拥有相同的生化系统，但目前还不清楚灯笼和生物发光系统是否有共同的起源。为了更好地了解上光组织和非上光组织的分子分化以及这些组织的分子生理学，我们比较了粗毛鞭毛虫(Phrixothrix Hirtus)和非发光黄毛虫(Chauliogna Flavipe)腹部侧灯和脂肪体的转录谱。我们在所有组织中都观察到了与信息素生物合成有关的基因产物，并观察到了四种不同的视蛋白基因产物，这比预期的要多，与灯夜蛾眼睛的转录分析相比。我们还观察到了几个可能与生物发光相关的基因产物，而在马尾松毛虫的脂肪体中，我们只发现了一个荧光素酶同源酶的拷贝。综上所述，这些结果表明，凤头花灯的上光组织与萤火虫花灯有着截然不同的起源，并且在一些Elateroidae科的脂肪体组织中也存在潜在的生物发光潜力。(C)爱思唯尔公司出版的2017年。
Bioluminescence in beetles is found in the Elateroidea superfamily, which includes Elateridae, Lampyridae, Rhagophthalmidae, and Phengodidae families. Despite sharing the same biochemical system, it is unclear whether the lanterns and the bioluminescent system share a common origin or not. To better understand the molecular differentiation of photogenic from non-photogenic tissues and the molecular physiology of these tissues, we compared the transcriptional profiles of the lateral lanterns and fat body in Phrixothrix hirtus railroad worm (Phengodidae) and the abdomen of the non-luminescent Chauliognathus flavipes (Cantharidae). We observed gene products involved in pheromone biosynthesis in all tissues and four distinct opsin gene products in C. flavipes, more than expected when compared to the transcriptional analysis of lampyrid eyes. We also observed several gene products potentially associated with bioluminescence in P. hirtus lantern and in C. flavipes body, whereas in the fat body of P. hirtus we found only a single copy of luciferase homolog enzyme. Altogether these results suggest that the photogenic tissue of phengodid lanterns has distinct origin from fireflies lanterns, and also the existence of a latent bioluminescent potential in the fat body tissue of some Elateroidea families. (C) 2017 Published by Elsevier Inc.